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S100B ELISA Kit (ab234573)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 139 pg/ml
  • Range: 0.31 ng/ml - 20 ng/ml
  • Sample type: Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    S100B ELISA Kit
    See all S100 beta kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Protein 6 2.5%

  • Sample type

    Tissue Extracts

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    139 pg/ml

  • Range

    0.31 ng/ml - 20 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Tissue Extracts 97 92% - 100%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Product overview

    S100B ELISA Kit (ab234573) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of S100B protein in tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate S100B with 139 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BI 1 x 6ml
    10X Human S100B Capture Antibody 1 x 600µl
    10X Human S100B Detector Antibody 1 x 600µl
    Human S100B Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling.

  • Tissue specificity

    Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.

  • Sequence similarities

    Belongs to the S-101 family.
    Contains 2 EF-hand domains.

  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

  • Alternative names

    • NEF
    • Protein S100 B
    • Protein S100-B
    • S 100 calcium binding protein beta chain
    • S 100 protein beta chain
    • S-100 protein beta chain
    • S-100 protein subunit beta
    • S100
    • S100 calcium binding protein beta (neural)
    • S100 calcium-binding protein B
    • S100 protein beta chain
    • S100B
    • S100B_HUMAN
    • S100beta
    see all

  • Database links

    see all

Images

  • Other - S100B ELISA Kit (ab234573)
    Other - S100B ELISA Kit (ab234573)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of S100B standard curve in 1X Cell Extraction Buffer PTR.
    Example of S100B standard curve in 1X Cell Extraction Buffer PTR.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native S100B protein in human, mouse, and rat brain extracts based on 2 µg/mL, 60 µg/mL, and 1 µg/mL loads, respectively.
    Interpolated concentrations of native S100B protein in human, mouse, and rat brain extracts based on 2 µg/mL, 60 µg/mL, and 1 µg/mL loads, respectively.

    The concentrations of S100B were measured in duplicates, interpolated from the S100B standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Sandwich ELISA - S100B ELISA Kit (ab234573)
    Sandwich ELISA - S100B ELISA Kit (ab234573)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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