Recombinant human CTLA4 protein (Active) (ab167727)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, ELISA, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituent: 99% PBS
Lyophilized from 0.22 µm filtered solution.
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
Images
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SDS-PAGE - Recombinant Human CTLA4 protein (ab167727)
SDS-PAGE of reduced ab167727 stained overnight with Coomassie Blue. The protein migrates as 25-30 kDa under reducing conditions due to glycosylation.
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ELISA - Recombinant Human CTLA4 protein (ab167727)Immobilized Human B7-1, Fc Tag (ab173993) at 2μg/mL (100 μL/well) can bind ab167727 with a linear range of 0.16-2.56 ng/mL.
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ELISA - Recombinant Human CTLA4 protein (ab167727)Immobilized Human B7-2, Fc Tag (ab167720) at 2μg/mL (100 μL/well) can bind ab167727 with a linear range of 1-6.4 ng/mL.
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Functional Studies - Recombinant Human CTLA4 protein (ab167727)Yervoy (Ipilimumab, Human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind ab167727 with an affinity constant of 25.7 nM as determined in SPR assay.
