Recombinant human Cleaved Caspase-3 protein (ab198447)
Key features and details
- Expression system: Escherichia coli
- Purity: > 99% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human Cleaved Caspase-3 protein
See all Cleaved Caspase-3 proteins and peptides -
Biological activity
Specific Activity: ≥ 6,784 pmol/min/µg
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Purity
> 99 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MENTENSVDSKSIKNLEPKIIHGSESMDSGISLDNSYKMDYPEMGLCIII NNKNFHKSTGMTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVELM RDVSKEDHSKRSSFVCVLLSHGEEGIIFGTNGPVDLKKITNFFRGDRCRS LTGKPKLFIIQACRGTELDCGIETDSGVDDDMACHKIPVEADFLYAYSTA PGYYSWRNSKDGSWFIQSLCAMLKQYADKLEFMHILTRVNRKVATEFESF SFDATFHAKKQIPCIVSMLTKELYFYHHHHHHH -
Predicted molecular weight
32 kDa including tags -
Amino acids
1 to 277 -
Tags
His tag C-Terminus -
Additional sequence information
Procaspase-3 is self-cleaved into two subunits, MW = 17 kDa and 13 kDa, to form the active heterotetrameric complex.
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant human Cleaved Caspase-3 protein (ab198447)
4-20% SDS-PAGE analysis of ab198447 (2 μg) with Coomassie staining.
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Functional Studies - Recombinant human Cleaved Caspase-3 protein (ab198447)Example of specific activity of ab198447.