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Immunology Innate Immunity Macrophage / Inflamm.

Recombinant Human CHIT1 protein (ab151883)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE, HPLC

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Description

  • Product name

    Recombinant Human CHIT1 protein
  • Purity

    > 95 % SDS-PAGE.
    Purity is greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. ab151883 is supplied as a 0.2 µm filtered solution.
  • Endotoxin level

  • Expression system

    HEK 293 cells
  • Accession

    Q13231
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      AKLVCYFTNWAQYRQGEARFLPKDLDPSLCTHLIYAFAGMTNHQLSTTEW NDETLYQEFNGLKKMNPKLKTLLAIGGWNFSTQKFTDMVATANNRQTFVN SAIRFLRKYSFDGLDLDWEYPGSQGSPAVDKERFTTLVQDLANAFQQEAQ TSGKERLLLSAAVPAGQTYVDAGYEVDKIAQNLDFVNLMAYDFHGSWEKV TGHNSPLYKRQEESGAAASLNVDAAVQQWLQKGTPASKLILGMPTYGRSF TLASSSDTRVGAPATGSGTPGPFTKEGGMLAYYEVCSWKGATKQRIQDQK VPYIFRDNQWVGFDDVESFKTKVSYLKQKGLGGAMVWALDLDDFAGFSCN QGRYPLIQTLRQELSLPYLPSGTPELEVPKPGQPSEPEHGPSPGQDTFCQ GKADGLYPNPRERSSFYSCAGGRLFQQSCPTGLVFSNSCKCCTWNVDHHH HHH
    • Predicted molecular weight

      50 kDa including tags
    • Amino acids

      22 to 466
    • Tags

      His tag C-Terminus
  • Associated products

    • Related Products

      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-6X His tag® antibody (ab9108)
      • Anti-CHIT1 antibody (ab96045)

    Specifications

    Our Abpromise guarantee covers the use of ab151883 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      HPLC

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 7.50
      Constituents: 0.32% Tris HCl, 0.88% Sodium chloride

    General Info

    • Alternative names

      • CHI3
      • CHIT
      • CHIT1
      • CHIT1_HUMAN
      • CHITD
      • Chitinase 1
      • Chitinase 1 (chitotriosidase)
      • Chitinase-1
      • Chitotriosidase
      • Chitotriosidase-1
      • EC 3.2.1.14
      • FLJ00314
      • MGC125322
      • Plasma methylumbelliferyl tetra N acetylchitotetraoside hydrolase
      see all
    • Function

      Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity.
    • Tissue specificity

      Detected in spleen. Secreted by cultured macrophages.
    • Sequence similarities

      Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.
      Contains 1 chitin-binding type-2 domain.
    • Cellular localization

      Secreted. Lysosome. A small proportion is lysosomal.
    • Target information above from: UniProt accession Q13231 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab151883? Please let us know so that we can cite the reference in this datasheet.

    ab151883 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 7.50
      Constituents: 0.32% Tris HCl, 0.88% Sodium chloride

    Images

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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