Recombinant Human CHD4 protein (His tag) (ab275792)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Endotoxin level:
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
-
Product name
Recombinant Human CHD4 protein (His tag) -
Purity
> 90 % SDS-PAGE. -
Endotoxin level
Expression system
Escherichia coliAccession
Protein length
Protein fragmentAnimal free
NoNature
Recombinant-
Species
Human -
Sequence
KDLNDEKQKK NIKQRFMFNI ADGGFTELHS LWQNEERAAT VTKKTYEIWH RRHDYWLLAG IINHGYARWQ DIQNDPRYAI LNEPFKGEMN RGNFLEIKNK FLARRFKLLE QALVIEEQLR RAAYLNMSED PSHPSMALNT RFAEVECLAE SHQHLSKESM AGNKPANAVL HKVLKQLEEL LSDMKADVTR LPATIARIPP VAVRLQMSER NILSRLANRA PEPTPQQVAQ QQ -
Predicted molecular weight
31 kDa -
Molecular weight information
32 kDa by SDS-PAGE -
Amino acids
1681 to 1912 -
Tags
His tag N-Terminus
Specifications
Our Abpromise guarantee covers the use of ab275792 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
SDS-PAGE
-
Form
Lyophilized -
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8
Constituents: 0.24% Tris, 0.87% Sodium chloride, 0.01% Sarkosyl, 5% Trehalose -
ReconstitutionReconstitute in PBS.
General Info
-
Alternative names
- ATP dependent helicase CHD4
- ATP-dependent helicase CHD4
- CHD 4
see all -
Function
Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. -
Sequence similarities
Belongs to the SNF2/RAD54 helicase family.
Contains 2 chromo domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 2 PHD-type zinc fingers. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase. - Information by UniProt
Images
-
SDS-PAGE - Recombinant Human CHD4 protein (His tag) (ab275792)
SDS-PAGE analysis of ab275792.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab275792 has not yet been referenced specifically in any publications.
Preparation and Storage
- ATP dependent helicase CHD4
- ATP-dependent helicase CHD4
- CHD 4
Images
-
SDS-PAGE - Recombinant Human CHD4 protein (His tag) (ab275792)
SDS-PAGE analysis of ab275792.