Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant Human CHD4 protein (His tag) (ab275792)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 90% SDS-PAGE
  • Endotoxin level:
  • Tags: His tag N-Terminus
  • Suitable for: SDS-PAGE

Description

  • Product name

    Recombinant Human CHD4 protein (His tag)

  • Purity

    > 90 % SDS-PAGE.

  • Endotoxin level

  • Expression system

    Escherichia coli

  • Accession

    Q14839

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      KDLNDEKQKK NIKQRFMFNI ADGGFTELHS LWQNEERAAT VTKKTYEIWH RRHDYWLLAG IINHGYARWQ DIQNDPRYAI LNEPFKGEMN RGNFLEIKNK FLARRFKLLE QALVIEEQLR RAAYLNMSED PSHPSMALNT RFAEVECLAE SHQHLSKESM AGNKPANAVL HKVLKQLEEL LSDMKADVTR LPATIARIPP VAVRLQMSER NILSRLANRA PEPTPQQVAQ QQ

    • Predicted molecular weight

      31 kDa

    • Molecular weight information

      32 kDa by SDS-PAGE

    • Amino acids

      1681 to 1912

    • Tags

      His tag N-Terminus

    • Specifications

    Our Abpromise guarantee covers the use of ab275792 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

      pH: 8
      Constituents: 0.24% Tris, 0.87% Sodium chloride, 0.01% Sarkosyl, 5% Trehalose

    • Reconstitution
      Reconstitute in PBS.

    General Info

    • Alternative names

      • ATP dependent helicase CHD4
      • ATP-dependent helicase CHD4
      • CHD 4
      • CHD-4
      • CHD4
      • CHD4_HUMAN
      • Chromodomain helicase DNA binding protein 4
      • Chromodomain-helicase-DNA-binding protein 4
      • Mi 2 autoantigen 218 kDa protein
      • Mi 2b
      • Mi-2 autoantigen 218 kDa protein
      • Mi2 beta
      • Mi2-beta
      see all

    • Function

      Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.

    • Sequence similarities

      Belongs to the SNF2/RAD54 helicase family.
      Contains 2 chromo domains.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 2 PHD-type zinc fingers.

    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • Cellular localization

      Nucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase.
    • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human CHD4 protein (His tag) (ab275792)
    SDS-PAGE - Recombinant Human CHD4 protein (His tag) (ab275792)

    SDS-PAGE analysis of ab275792.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab275792? Please let us know so that we can cite the reference in this datasheet.

ab275792 has not yet been referenced specifically in any publications.

Preparation and Storage

  • ATP dependent helicase CHD4
  • ATP-dependent helicase CHD4
  • CHD 4

Images

  • SDS-PAGE - Recombinant Human CHD4 protein (His tag) (ab275792)
    SDS-PAGE - Recombinant Human CHD4 protein (His tag) (ab275792)

    SDS-PAGE analysis of ab275792.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Clear all

Recently viewed products

Sign up