Recombinant Human CDK13 + CyclinK protein (ab204098)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% Densitometry
- Tags: GST tag N-Terminus, GST tag N-Terminus
- Suitable for: WB, SDS-PAGE
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Product name
Recombinant Human CDK13 + CyclinK protein -
Purity
> 70 % Densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Sequence
KKIKIEHAPSPSSGGTLKNDKAKTKPPLQVTKVENNLIVDKATKKAVIVG KESKSAATKEESVSLKEKTKPLTPSIGAKEKEQHVALVTSTLPPLPLPPM LPEDKEADSLRGNISVKAVKKEVEKKLRCLLADLPLPPELPGGDDLSKSP EEKKTATQLHSKRRPKICGPRYGETKEKDIDWGKRCVDKFDIIGIIGEGT YGQVYKARDKDTGEMVALKKVRLDNEKEGFPITAIREIKILRQLTHQSII NMKEIVTDKEDALDFKKDKGAFYLVFEYMDHDLMGLLESGLVHFNENHIK SFMRQLMEGLDYCHKKNFLHRDIKCSNILLNNRGQIKLADFGLARLYSSE ESRPYTNKVITLWYRPPELLLGEERYTPAIDVWSCGCILGELFTKKPIFQ ANQELAQLELISRICGSPCPAVWPDVIKLPYFNTMKPKKQYRRKLREEFV FIPAAALDLFDYMLALDPSKRCTAEQALQCEFLRDVEPSKMPPPDLPLWQ DCHELWSKKRRRQKQMGMTDDVSTIKAPRKDLSLGLDDSRTNTPQGVLPS SQLKSQGSSNVAPVKTGPGQHLNHSELAILLNLLQSKTSVNMADFVQVLN IKVNSETQQQLNKINLPAGILATGEKQTDPSTPQQESSKPLGGIQPSSQT IQPKVETDAAQAAVQSAFAVLLTQLIKAQQSKQKDVLLEERENGSGHEAS LQLRPPPEPSTPVSGQDDLIQHQDMRILELTPEPDRPRILPPDQRPPEPP EPPPVTEEDLDYRTENQHVPTTSSSLTDPHAGVKAALLQLLAQHQPQDDP KREGGIDYQAGDTYVSTSDYKDNFGSSSFSSAPYVSNDGLGSSSAPPLER RSFIGNSDIQSLDNYSTASSHSGGPPQPSAFSESFPSSVAGYGDIYLNAG PMLFSGDKDHRFEYSHGPIAVLANSSDPSTGPESTHPLPAKMHNYNYGGN LQENPSGPSLMHGQTWTSPAQGPGYSQGYRGHISTSTGRGRGRGLPY -
Predicted molecular weight
160 kDa including tags -
Amino acids
516 to 1512 -
Tags
GST tag N-Terminus
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Amino Acid Sequence 2
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Species
Human -
Sequence
MKENKENSSPSVTSANLDHTKPCWYWDKKDLAHTPSQLEGLDPATEARYR REGARFIFDVGTRLGLHYDTLATGIIYFHRFYMFHSFKQFPRYVTGACCL FLAGKVEETPKKCKDIIKTARSLLNDVQFGQFGDDPKEEVMVLERILLQT IKFDLQVEHPYQFLLKYAKQLKGDKNKIQKLVQMAWTFVNDSLCTTLSLQ WEPEIIAVAVMYLAGRLCKFEIQEWTSKPMYRRWWEQFVQDVPVDVLEDI CHQILDLYSQGKQQMPHHTPHQLQQPPSLQPTPQVPQVQQSQPSQSSEPS QPQQKDPQQPAQQQQPAQQPKKPSPQPSSPRQVKRAVVVSPKEENKAAEP PPPKIPKIETTHPPLPPAHPPPDRKPPLAAALGEAEPPGPVDATDLPKVQ IPPPAHPAPVHQPPPLPHRPPPPPPSSYMTGMSTTSSYMSGEGYQSLQSM MKTEGPSYGALPPAYGPPAHLPYHPHVYPPNPPPPPVPPPPASFPPPAIP PPTPGYPPPPPTYNPNFPPPPPRLPPTHAVPPHPPPGLGLPPASYPPPAV PPGGQPPVPPPIPPPGMPPVGGLGRAAWMR -
Predicted molecular weight
67 kDa including tags -
Amino acids
1 to 580 -
Tags
GST tag N-Terminus
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Preparation and Storage
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Alternative names
- CCNK
- CDC2L
- CDC2L5
see all -
Relevance
Cyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. Has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. Required for RNA splicing, probably by phosphorylating SRSF1/SF2. Required during hematopoiesis. In case of infection by HIV-1 virus, interacts with HIV-1 Tat protein acetylated at 'Lys-50' and 'Lys-51', thereby increasing HIV-1 mRNA splicing and promoting the production of the doubly spliced HIV-1 protein. Cyclin K regulatory subunit of cyclin-dependent kinases that mediates activation of target kinases. Plays a role in transcriptional regulation via its role in regulating the phosphorylation of the C-terminal domain (CTD) of the large subunit of RNA polymerase II -
Cellular localization
Nucleus speckle.
Images
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SDS-PAGE - Recombinant Human CDK13 + CyclinK protein (ab204098)
SDS-PAGE showing ab204098.