Brilliant Cresyl Blue Solution (1.5%) ab246814 is a supravital hematology stain for counting reticulocytes in peripheral blood smears. The number of reticulocytes in the blood is a simple measure of erythropoietic performance. Reticulocytes are immature erythrocytes that contain nuclear remnants of basophilic ribonucleoproteins which decreases as the reticulocyte matures. When stained, reticulocytes appear to contain dark blue granules or filaments.

Staining Interpretation:

Reticulocytes: Blue/Green with Dark Blue Granules.

Erythrocytes: Blue/Green.

Leukocytes: Light Blue.

Control Tissue: Blood.

Other products for staining tissue sections

Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

Procedure (Glass Tube): 

1. Pipette a volume of Brilliant Cresyl Blue in the bottom of a glass tube followed by an equal volume of uncoagulated blood and mix well.

2. Incubate mixture for 5-15 minutes in a warm water bath.

3. Mix well prior to preparing smear.

4. Prepare 2-3 glass slide smears and let dry.

5. Clear slide and mount in synthetic resin.

Procedure (Glass Slide): 

1. Pipette a volume of Brilliant Cresyl Blue in the bottom of a glass slide followed by an equal volume of uncoagulated blood and mix well.

2. Place immediately in a warm humidity chamber and incubate mixture for 5-15 minutes.

3. Mix well prior to preparing smear.

4. Prepare smear and let dry.

5. Clear slide and mount in synthetic resin.