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Immunology Adaptive Immunity T Cells Cytotoxic Cells

Recombinant Human CD8 alpha + CD8B protein (Tagged) (ab277034)

Recombinant Human CD8 alpha + CD8B protein (Tagged) (ab277034)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE

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Description

  • Product name

    Recombinant Human CD8 alpha + CD8B protein (Tagged)
  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

  • Expression system

    HEK 293 cells
  • Accession

    P01732
    P10966
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Amino Acid Sequence 1

    • Species

      Human
    • Sequence

      MALPVTALLL PLALLLHAAR PSQFRVSPLD RTWNLGETVE LKCQVLLSNP TSGCSWLFQP RGAAASPTFL LYLSQNKPKA AEGLDTQRFS GKRLGDTFVL TLSDFRRENE GYYFCSALSN SIMYFSHFVP VFLPAKPTTT PAPRPPTPAP TIASQPLSLR PEACRPAAGG AVHTRGLDFA CD
    • Predicted molecular weight

      22 kDa including tags
    • Molecular weight information

      The recombinant heterodimer of human CD8A&CD8B consists of 392 (202+190) amino acids and has a calculated molecular mass of 44.1 (22.4+21.7) KDa.
    • Amino acids

      1 to 182
    • Additional sequence information

      FLAG tag at C-terminus.
    • Amino Acid Sequence 2

    • Species

      Human
    • Sequence

      MRPRLWLLLA AQLTVLHGNS VLQQTPAYIK VQTNKMVMLS CEAKISLSNM RIYWLRQRQA PSSDSHHEFL ALWDSAKGTI HGEEVEQEKI AVFRDASRFI LNLTSVKPED SGIYFCMIVG SPELTFGKGT QLSVVDFLPT TAQPTKKSTL KKRVCRLPRP ETQKGPLCSP
    • Predicted molecular weight

      22 kDa including tags
    • Amino acids

      1 to 170
    • Tags

      His tag C-Terminus
  • Specifications

    Our Abpromise guarantee covers the use of ab277034 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at Room Temperature. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.40
      Constituent: 100% PBS

    • Reconstitution
      This information is lot specific. Please contact our technical Support team for details.

    General Info

    • Cellular localization

      CD8 alpha: Secreted and Cell membrane. CD8B: Isoform 1: Cell membrane; Single-pass type I membrane protein (Probable). Isoform 2: Cell membrane; Single-pass type I membrane protein (Probable). Isoform 3: Secreted (Probable). Isoform 4: Cell membrane; Single-pass type I membrane protein (Probable). Isoform 5: Cell membrane; Single-pass type I membrane protein (Probable). Isoform 6: Secreted (Probable). Isoform 7: Secreted (Probable). Isoform 8: Secreted (Probable).

Images

  • SDS-PAGE - Recombinant Human CD8 alpha + CD8B protein (ab277034)
    SDS-PAGE - Recombinant Human CD8 alpha + CD8B protein (ab277034)
    SDS-PAGE analysis of ab277034

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab277034? Please let us know so that we can cite the reference in this datasheet.

    ab277034 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • Cellular localization

      CD8 alpha: Secreted and Cell membrane. CD8B: Isoform 1: Cell membrane; Single-pass type I membrane protein (Probable). Isoform 2: Cell membrane; Single-pass type I membrane protein (Probable). Isoform 3: Secreted (Probable). Isoform 4: Cell membrane; Single-pass type I membrane protein (Probable). Isoform 5: Cell membrane; Single-pass type I membrane protein (Probable). Isoform 6: Secreted (Probable). Isoform 7: Secreted (Probable). Isoform 8: Secreted (Probable).

    Images

    • SDS-PAGE - Recombinant Human CD8 alpha + CD8B protein (ab277034)
      SDS-PAGE - Recombinant Human CD8 alpha + CD8B protein (ab277034)
      SDS-PAGE analysis of ab277034

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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