Recombinant human CD73 protein (Active) (ab271449)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human CD73 protein (Active)
See all CD73 proteins and peptides -
Biological activity
Specific activity >20 nmol/mon/μg.
Assay was done in 50 µL assay buffer containing 25 mM Tris, pH 7.4, 5 mM MgCl2 and 100 µM AMP for 12 min at 37º C. Inorganic phosphate produced was measured using Biomol Green reagent.
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Purity
>= 90 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
WELTILHTND VHSRLEQTSE DSSKCVNASR CMGGVARLFT KVQQIRRAEP NVLLLDAGDQ YQGTIWFTVY KGAEVAHFMN ALRYDAMALG NHEFDNGVEG LIEPLLKEAK FPILSANIKA KGPLASQISG LYLPYKVLPV GDEVVGIVGY TSKETPFLSN PGTNLVFEDE ITALQPEVDK LKTLNVNKII ALGHSGFEMD KLIAQKVRGV DVVVGGHSNT FLYTGNPPSK EVPAGKYPFI VTSDDGRKVP VVQAYAFGKY LGYLKIEFDE RGNVISSHGN PILLNSSIPE DPSIKADINK WRIKLDNYST QELGKTIVYL DGSSQSCRFR ECNMGNLICD AMINNNLRHT DEMFWNHVSM CILNGGGIRS PIDERNNGTI TWENLAAVLP FGGTFDLVQL KGSTLKKAFE HSVHRYGQST GEFLQVGGIH VVYDLSRKPG DRVVKLDVLC TKCRVPSYDP LKMDEVYKVI LPNFLANGGD GFQMIKDELL RHDSGDQDIN VVSTYISKMK VIYPAVEGRI K -
Predicted molecular weight
59 kDa including tags -
Molecular weight information
Protein runs at higher than predicted MW on SDS-PAGE due to glycosylation. -
Amino acids
27 to 547 -
Tags
His tag C-Terminus -
Additional sequence information
Genbank: NM_002526
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Preparation and Storage
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Alternative names
- 5' NT
- 5' nucleotidase (CD73)
- 5' nucleotidase precursor
see all -
Function
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. -
Involvement in disease
Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands. -
Sequence similarities
Belongs to the 5'-nucleotidase family. -
Cellular localization
Cell membrane. - Information by UniProt
Images
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Functional Studies - Recombinant Human CD73 protein (Tagged) (ab271449)
Functional analysis of ab271449.
Specific activity >20 nmol/mon/μg.
Assay was done in 50 µL assay buffer containing 40 mM Tris, pH 7.4, 20 mM MgCl2, 0.1 mg/ml BSA and 40 µM AMP for 20 min at 37°C.
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SDS-PAGE - Recombinant Human CD73 protein (Tagged) (ab271449)SDS-PAGE analysis of ab271449.