Recombinant human CD44 protein (Active) (ab221334)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: ELISA, HPLC, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: 0.61% Tris, Glycine, Trehalose, L-Arginine, Sodium chloride
Lyophilized from 0.22 µm filtered solutionThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
Images
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ELISA - Recombinant Human CD44 protein (Fc Chimera) (ab221334)
Immobilized ab221334 at 5 μg/mL (100 μL/well) can bind Hyaluronan biotin sodium salt with a linear range of 10-156 ng/mL.
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SDS-PAGE - Recombinant Human CD44 protein (Fc Chimera) (ab221334)SDS-PAGE of reduced ab221334 stained overnight with Coomassie Blue. Due to glycosylation, the protein migrates at 76 kDa on a under reducing conditions.
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ELISA - Recombinant Human CD44 protein (Fc Chimera) (ab221334)Immobilized Hyaluronic Acid at 100 μg/mL (100 μL/well) can bind ab221334 with a linear range of 0.156-2.5 μg/mL.
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HPLC - Recombinant Human CD44 protein (Fc Chimera) (ab221334)SEC-HPLC analysis of ab221334. The purity of ab221334 was determined to be greater than 90%.
