Recombinant human CD19 protein (Active) (ab234966)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA). Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C..
pH: 7.40
Constituents: 5% Trehalose, PBS
Lyophilized from 0.22 µm filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
Images
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ELISA - Recombinant human CD19 protein (Active) (ab234966)
Immobilized FMC63 MAb at 2 μg/mL (100 μL/well) binds ab234699.
Linear range: 10-78 ng/mL (QC tested).
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ELISA - Recombinant human CD19 protein (Active) (ab234966)ab234966 at 2 μg/mL (100 μL/well) on Nickel Coated Plate, binds FMC63 (Mouse IgG2a).
Linear range: 2-13 ng/mL (Routinely tested).
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ELISA - Recombinant human CD19 protein (Active) (ab234966)ab234966 at 0.1 μg/mL (100 μL/well) on MonRabTM Anti-His Tag (C-term) Antibody precoated (0.1 μg/well) plate, binds FMC63 (Mouse IgG2a).
Linear range: 0.6-10 ng/mL (Routinely tested).
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Functional Studies - Recombinant human CD19 protein (Active) (ab234966)Captured Human CD19 (20-291), His Tag on CM5 Chip via anti-His antibody, can bind FMC63 (mouse lgG2a) with an affinity constant of 2.01 nM as determined in SPR assay (Biacore T200 ). -
Functional Studies - Recombinant human CD19 protein (Active) (ab234966)FMC63 MAb (mouse lgG2a) captured on CM5 chip via anti-mouse antibodies surface, binds ab234966.
Affinity constant of 2.95 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
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SDS-PAGE - Recombinant human CD19 protein (Active) (ab234966)Reduced ab234966 on SDS-PAGE, stained overnight with Coomassie Blue.
Purity of protein >90%
The protein migrates as 45-50 kDa under reducing conditions due to glycosylation.
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Flow Cytometry - Recombinant human CD19 protein (Active) (ab234966)293 cells were transfected with FMC63-scFv and RFP tag.
2x105 of the cells were first incubated with:
A. His Tag-protein control.
B. 10 ug/ml ab234966
C. 10 ug/ml ab234966 and FMC63 (Mouse anti-CD19 antibody).
The FITC Anti-6xHis tag antibody was used to analyse with FACS.
RFP was used to evaluate CAR (FMC63-scFv) expression and FITC was used to evaluate the binding activity ofab234966.
