Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Expression system: Escherichia coli
Purity: > 95% SDS-PAGE
Endotoxin level:
Tags: His tag C-Terminus
Suitable for: HPLC, SDS-PAGE

Product name

Recombinant Human BLK protein
See all BLK proteins and peptides
Purity

> 95 % SDS-PAGE.
ab172848 is greater than 95% pure, as determined by SEC-HPLC and reducing SDS-PAGE. It is supplied as an 0.2 µM filtered solution.
Endotoxin level
Expression system

Escherichia coli
Accession

P51451
Protein length

Full length protein
Animal free

No
Nature

Recombinant
- Species
  
  Human
- Sequence
  
  MGLVSSKKPDKEKPIKEKDKGQWSPLKVSAQDKDAPPLPPLVVFNHLTPP PPDEHLDEDKHFVVALYDYTAMNDRDLQMLKGEKLQVLKGTGDWWLARSL VTGREGYVPSNFVARVESLEMERWFFRSQGRKEAERQLLAPINKAGSFLI RESETNKGAFSLSVKDVTTQGELIKHYKIRCLDEGGYYISPRITFPSLQA LVQHYSKKGDGLCQRLTLPCVRPAPQNPWAQDEWEIPRQSLRLVRKLGSG QFGEVWMGYYKNNMKVAIKTLKEGTMSPEAFLGEANVMKALQHERLVRLY AVVTKEPIYIVTEYMARGCLLDFLKTDEGSRLSLPRLIDMSAQIAEGMAY IERMNSIHRDLRAANILVSEALCCKIADFGLARIIDSEYTAQEGAKFPIK WTAPEAIHFGVFTIKADVWSFGVLLMEVVTYGRVPYPGMSNPEVIRNLER GYRMPRPDTCPPELYRGVIAECWRSRPEERPTFEFLQSVLEDFYTATERQ YELQPLE
- Predicted molecular weight
  
  59 kDa including tags
- Amino acids
  
  1 to 505
- Tags
  
  His tag C-Terminus

Our Abpromise guarantee covers the use of ab172848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Applications

HPLC

SDS-PAGE
Form

Liquid
Concentration information loading...

Stability and Storage

Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

pH: 7.40
Constituents: 0.24% Tris, 2.9% Sodium chloride, 0.02% DTT

Alternative names
- B lymphocyte kinase
- B lymphoid tyrosine kinase
- Blk
- BLK nonreceptor tyrosine kinase
- BLK_HUMAN
- EC 2.7.10.2
- MGC 10442
- MGC10442
- MODY 11
- MODY11
- P55 BLK
- p55-BLK
- Tyrosine kinase B lymphocyte specific
- Tyrosine protein kinase
- Tyrosine-protein kinase BLK
see all
Function

Modulator of beta-cells function, acting through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose.
Tissue specificity

Expressed in lymphatic organs, pancreatic islets, Leydig cells, striate ducts of salivary glands and hair follicles.
Involvement in disease

Defects in BLK are a cause of maturity-onset diabetes of the young type 11 (MODY11) [MIM:613375]. MODY11 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
Target information above from: UniProt accession P51451 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols