Recombinant B. pertussis Adenylate Cyclase protein (ab179866)
Key features and details
- Expression system: Escherichia coli
- Purity: > 80% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, WB, SDS-PAGE, ELISA
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Product name
Recombinant B. pertussis Adenylate Cyclase protein -
Biological activity
Addition of 10~100 ng/ml of ab179866 to medium significantly increased the levels of cellular cAMP levels in CHO and human monocyte cells.
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Purity
> 80 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Bordetella pertussis -
Sequence
MQQSHQAGYANAADRESGIPAAVLDGIKAVAKEKNATLMFRLVNPHSTSL IAEGVATKGLGVHAKSSDWGLQAGYIPVNPNLSKLFGRAPEVIARADNDV NSSLAHGHTAVDLTLSKERLDYLRQAGLVTGMADGVVASNHAGYEQFEFR VKETSDGRYAVQYRRKGGDDFEAVKVIGNAAGIPLTADIDMFAIMPHLSN FRDSARSSVTSGDSVTDYLARTRRAASEATGGLDRERIDLLWKIARAGAR SAVGTEARRQFRYDGDMNIGVITDFELEVRNALNRRAHAVGAQDVVQHGT EQNNPFPEADEKIFVVSATGESQMLTRGQLKEYIGQQRGEGYVFYENRAY GVAGKSLFDDGLGAAPGVPSGRSKFSPDVLETVPASPGLRRPSLGAVERQ DSGYDSLDGVGSRSFSLGEVSDMAAVEAAELEMTRQVLHAGARQDDAEPG VSGASAHWGQRALQGAQAVAAAQRLVHAIALMTQFGRAGSTNTPQEAASL SAAVFGLGEASSAVAETVSGFFRGSSRWAGGFGVAGGAMALGGGIAAAVG AGMSLTDDAPAGQKAAAGAEIALQLTGGTVELASSIALALAAARGVTSGL QVAGASAGAAAGALAAALSPMEIYGLVQQSHYADQLDKLAQESSAYGYEG DALLAQLYRDKTAAEGAVAGVSAVLSTVGAAVSIAAAASVVGAPVAVVTS LLTGALNGILRGVQQPIIEKLANDYARKIDELGGPQAYFEKNLQARHEQL ANSDGLRKMLADLQAGWNASSVIGVQTTEISKSALELAAITGNADNLKSV DVFVDRFVQGERVAGQPVVLDVAAGGIDIASRKGERPALTFITPLAAPGE EQRRRTKTGKSEFTTFVEIVGKQDRWRIRDGAADTTIDLAKVVSQLVDAN GVLKHSIKLDVIGGDGDDVVLANASRIHYDGGAGTNTVSYAALGRQDSIT VSADGERFNVRKQLNNANVYREGVATQTTAYGKRTENVQYRHVELARVGQ LVEVDTLEHVQHIIGGAGNDSITGNAHDNFLAGGSGDDRLDGGAGNDTLV GGEGQNTVIGGAGDDVFLQDLGVWSNQLDGGAGVDTVKYNVHQPSEERLE RMGDTGIHADLQKGTVEKWPALNLFSVDHVKNIENLHGSRLNDRIAGDDQ DNELWGHDGNDTIRGRGGDDILRGGLGLDTLYGEDGNDIFLQDDETVSDD IDGGAGLDTVDYSAMIHPGRIVAPHEYGFGIEADLSREWVRKASALGVDY YDNVRNVENVIGTSMKDVLIGDAQANTLMGQGGDDTVRGGDGDDLLFGGD GNDMLYGDAGNDTLYGGLGDDTLEGGAGNDWFGQTQAREHDVLRGGDGVD TVDYSQTGAHAGIAAGRIGLGILADLGAGRVDKLGEAGSSAYDTVSGIEN VVGTELADRITGDAQANVLRGAGGADVLAGGEGDDVLLGGDGDDQLSGDA GRDRLYGEAGDDWFFQDAANAGNLLDGGDGRDTVDFSGPGRGLDAGAKGV FLSLGKGFASLMDEPETSNVLRNIENAVGSARDDVLIGDAGANVLNGLAG NDVLSGGAGDDVLLGDEGSDLLSGDAGNDDLFGGQGDDTYLFGVGYGHDT IYESGGGHDTIRINAGADQLWFARQGNDLEIRILGTDDALTVHDWYRDAD HRVEIIHAANQAVDQAGIEKLVEAMAQYPDPGAAAAAPPAARVPDTLMQS LAVNWR -
Predicted molecular weight
178 kDa -
Amino acids
1 to 1706 -
Additional sequence information
ab179866 also contains the cyaC activator gene product (aa 1-185; MWt 20kDa) (UniProt P0A3I5).
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Preparation and Storage
- AC-HLY
- ACT
- bifunctional hemolysin-adenylate cyclase
Images
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SDS-PAGE - Recombinant B. pertussis Adenylate Cyclase protein (ab179866)
8% SDS-PAGE analysis of ab179866.