Recombinant human Activin Receptor Type IIA/ACVR2A protein (Active) (Biotin) (ab271359)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: = 90% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus, Avi tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human Activin Receptor Type IIA/ACVR2A protein (Active) (Biotin)
See all Activin Receptor Type IIA/ACVR2A proteins and peptides -
Biological activity
Kinase assay using casein as substrate.
Enzyme reactions include 40 mM Tris pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA, 10 μM ATP, 0.2 mg/mL casein, 3 mM DTT, and various amounts of ab271359—incubated 45 minutes at 30 °C. Followed by addition of 25 μL of luminescent reagent—incubated 45 minutes at room temperature. Followed by addition of 50 µl of proprietary detection reagent —incubated 45 minutes at room temperature. Luminescence was measured.
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Purity
= 90 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
45 kDa including tags -
Amino acids
147 to 509 -
Tags
DDDDK tag N-Terminus , Avi tag C-Terminus
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Conjugation
Biotin
Preparation and Storage
- Activin A receptor type IIA
- Activin receptor type 2A
- Activin receptor type IIA
Images
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Functional Studies - (ab271359)
Kinase activity of ab271359 using casein as substrate.
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SDS-PAGE - (ab271359)SDS-PAGE analysis of ab271359.
