Recombinant HIV1 Nef protein (ab185584)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His-T7 tag N-Terminus
- Suitable for: SDS-PAGE
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Product name
Recombinant HIV1 Nef protein
See all HIV1 Nef proteins and peptides -
Purity
> 90 % SDS-PAGE.
The final product was refolded using temperature shift inclusion body refolding technology and chromatographically purified. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Sequence
MASMTGGQQMGRGHHHHHHENLYFQGGFGGKWSKSSVIGWPAVRERMRRA EPAADGVGAVSRDLEKHGAITSSNTAANNAACAWLEAQEEEEVGFPVTPQ VPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQRRQDILDLWIYHTQGYFPD WQNYTPGPGVRYPLTFGWCYKLVPVEPDKVEEANKGENTSLLHPVSLHGM DDPEREVLEWRFDSRLAFHHVARELHPEYFKNC -
Predicted molecular weight
26 kDa including tags -
Amino acids
1 to 206 -
Tags
His-T7 tag N-Terminus -
Additional sequence information
Derived from NL4-3 strain and constructed with codon optimization and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal.
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituent: 0.32% Tris HCl
Contains NaCl, KCl, EDTA, arginine, DTT and Sucrose.
