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Recombinant GP protein (ab199980)

Recombinant GP protein (ab199980)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 90% SDS-PAGE
  • Endotoxin level:
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE

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Description

  • Product name

    Recombinant GP protein
  • Purity

    > 90 % SDS-PAGE.

  • Endotoxin level

  • Expression system

    HEK 293 cells
  • Accession

    ACI28624
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Not applicable
    • Sequence

      IPLGVVHNNTLQVSDIDKLVCRDKLSSTSQLKSVGLNLEGNGVATDVPTA TKRWGFRAGVPPKVVNYEAGEWAENCYNLDIKKADGSECLPEAPEGVRGF PRCRYVHKVSGTGPCPEGYAFHKEGAFFLYDRLASTIIYRSTTFSEGVVA FLILPETKKDFFQSPPLHEPANMTTDPSSYYHTVTLNYVADNFGTNMTNF LFQVDHLTYVQLEPRFTPQFLVQLNETIYTNGRRSNTTGTLIWKVNPTVD TGVGEWAFWENKKNFTKTLSSE
    • Predicted molecular weight

      32 kDa including tags
    • Amino acids

      33 to 304
    • Tags

      His tag C-Terminus
  • Description

    Recombinant Not applicable GP protein (His tag)
  • Associated products

    • Related Products

      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-6X His tag® antibody (ab9108)

    Specifications

    Our Abpromise guarantee covers the use of ab199980 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Lyophilized
    • Additional notes

      This product is stable after storage at:

      • -20°C to -70°C for 12 months in lyophilized state;
      • -70°C for 3 months under sterile conditions after reconstitution.
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot. Upon reconsitution add a carrier protein (0.1% BSA). Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.

      pH: 7.4
      Constituent: 99% PBS

      5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is about 3-5%. Lyophilized from 0.22 µm filtered solution.

    • Reconstitution
      Reconstitute with sterile deionized water to a concentration of 200 µg/ml.

Images

  • SDS-PAGE - Recombinant GP protein (ab199980)
    SDS-PAGE - Recombinant GP protein (ab199980)

    The purity of ab199980 was determined by DTT-reduced SDS-PAGE and staining overnight with Coomassie Blue. DTT-reduced Protein migrates as 40-60 kDa in SDS-PAGE due to glycosylation.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab199980? Please let us know so that we can cite the reference in this datasheet.

    ab199980 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • -20°C to -70°C for 12 months in lyophilized state;
    • -70°C for 3 months under sterile conditions after reconstitution.

    Images

    • SDS-PAGE - Recombinant GP protein (ab199980)
      SDS-PAGE - Recombinant GP protein (ab199980)

      The purity of ab199980 was determined by DTT-reduced SDS-PAGE and staining overnight with Coomassie Blue. DTT-reduced Protein migrates as 40-60 kDa in SDS-PAGE due to glycosylation.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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