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Rat MMP3 ELISA Kit (ab270216)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 33.36 pg/ml
  • Range: 390.63 pg/ml - 25000 pg/ml
  • Sample type: Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Rat

Overview

  • Product name

    Rat MMP3 ELISA Kit
    See all MMP3 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 3%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 3.5%

  • Sample type

    Serum

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    33.36 pg/ml

  • Range

    390.63 pg/ml - 25000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 90 86% - 93%
    Hep Plasma 90 88% - 94%
    Cit plasma 93 89% - 97%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Rat

  • Product overview

    Rat MMP3 ELISA Kit (ab270216) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MMP3 protein in serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat MMP3 with 33.36 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Matrix metalloprotease-3 (MMP3), also known as Stromelysin-1, is a 54 kD secreted protease involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling. It also has known roles in disease processes, such as arthritis and cancer metastasis.  MMP3 functions by degrading fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans.  These actions are linked to activation of procollagenase to further help in the remodeling of the extracellular matrix environment. Rat MMP3 shares 76.2%, 90.2%, and 75.1% sequence homology with human, mouse, and cow, respectively.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Example of rat MMP3 standard curve in Sample Diluent NS.
    Example of rat MMP3 standard curve in Sample Diluent NS.
  • Interpolated concentrations of native MMP3 in rat serum and plasma samples.
    Interpolated concentrations of native MMP3 in rat serum and plasma samples.

    The concentrations of MMP3 were measured in duplicates, interpolated from the MMP3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP3 concentration was determined to be 66.05 ng/mL in serum, 73.89 ng/mL in plasma (citrate) and 56.23 ng/mL in plasma (heparin).

  • Sandwich ELISA - Rat MMP3 ELISA Kit (ab270216)
    Sandwich ELISA - Rat MMP3 ELISA Kit (ab270216)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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