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Rat Kininogen ELISA Kit (KNG) ELISA Kit (ab157742)

Key features and details

  • Sensitivity: 4.9707 ng/ml
  • Range: 6.25 ng/ml - 400 ng/ml
  • Sample type: Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Rat

Overview

  • Product name

    Rat Kininogen ELISA Kit (KNG) ELISA Kit
    See all Kininogen kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall
    Inter-assay
    Sample n Mean SD CV%
    Overall

  • Sample type

    Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    4.9707 ng/ml

  • Range

    6.25 ng/ml - 400 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 % - %

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Rat

  • Product overview

    Abcam’s Kininogen (KNG) Rat ELISA Kit is a two-site enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Kininogen levels in rat serum and plasma. 

     

    In this assay the Kininogen present in samples reacts with the anti-Kininogen antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Kininogen antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Kininogen. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo­genic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Kininogen in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Kininogen in the test sample. The quantity of Kininogen in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X HRP-conjugated anti-rat Kininogen antibody 1 x 150µl
    20X Wash Buffer Concentrate 1 x 50ml
    5X Diluent Concentrate 1 x 50ml
    Chromogen Substrate Solution 1 x 12ml
    Rat Kininogen Calibrator (Lyophilized) 1 vial
    Rat Kininogen ELISA Microplate 1 unit
    Stop Solution 1 x 12ml

  • Research areas

  • Function

    (1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.

  • Tissue specificity

    Secreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors.

  • Involvement in disease

    Defects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency) [MIM:228960]. HMWK deficiency is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis.

  • Sequence similarities

    Contains 3 cystatin domains.

  • Post-translational
    modifications

    Bradykinin is released from kininogen by plasma kallikrein.
    Hydroxylation of Pro-383 occurs prior to the release of bradykinin.
    Phosphorylation sites are present in the extracelllular medium.
    N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.

  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt

  • Alternative names

    • Alpha-2-thiol proteinase inhibitor
    • BDK
    • BK
    • BK, included
    • bradykinin
    • bradykinin, included
    • Fitzgerald factor
    • Fitzgerald factor, included
    • Flaujeac factor, included
    • High molecular weight kininogen
    • High molecular weight kininogen, included
    • HMWK
    • HMWK, included
    • Ile-Ser-Bradykinin
    • Kallidin I
    • Kallidin II
    • kininogen 1
    • KNG
    • KNG1
    • KNG1_HUMAN
    • LMWK, included
    • Low molecular weight growth-promoting factor
    • Low molecular weight kininogen, included
    • Williams factor, included
    • Williams-Fitzgerald-Flaujeac factor
    see all

  • Database links

    Images

    • Standard Curve
      Standard Curve
      Representative standard curve using ab157742 Kininogen Rat ELISA Kit

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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