Rat IL-4 ELISA Set (without plates) (ab48423)
Overview
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Product name
Rat IL-4 ELISA Set (without plates) -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
Range
1000 pg/ml - 31.25 pg/mlAssay duration
Multiple steps standard assaySpecies reactivity
Reacts with: RatProduct overview
Abcam’s IL-4 Rat in vitro ELISA Set is designed for the quantitative measurement of Rat IL-4 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
The rat IL-4 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
This ELISA Set is intended to measure rat IL-4 in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
Recognizes both natural and recombinant Rat IL-4 Range: 31.25 pg/ml - 1000 pg/ml Sensitivity
Tested applications
Suitable for: Sandwich ELISAmore detailsPlatform
ReagentsProperties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 5 x 96 tests 10 x 96 tests 15 x 96 tests Detection biotinylated anti-Rat IL-4 antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml Rat IL-4 Capture antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml Rat IL-4 Standard (Lyophilized) Yellow 5 vials 10 vials 15 vials Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials Streptavidin-HRP 1 vial 2 vials 3 vials -
Research areas
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Relevance
IL-4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL-4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL-4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. -
Cellular localization
Secreted -
Alternative names
- B cell stimulatory factor 1
- Binetrakin
- BSF1
see all -
Database links
- Entrez Gene: 287287 Rat
- SwissProt: P20096 Rat
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 5 x 96 tests 10 x 96 tests 15 x 96 tests Detection biotinylated anti-Rat IL-4 antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml Rat IL-4 Capture antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml Rat IL-4 Standard (Lyophilized) Yellow 5 vials 10 vials 15 vials Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials Streptavidin-HRP 1 vial 2 vials 3 vials -
Research areas
-
Relevance
IL-4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL-4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL-4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. -
Cellular localization
Secreted -
Alternative names
- B cell stimulatory factor 1
- Binetrakin
- BSF1
see all -
Database links
- Entrez Gene: 287287 Rat
- SwissProt: P20096 Rat
Images
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Rat IL-4 ELISA Set (ab48423)Rat IL-4 Standard curve ranging from 31.25 to 1000 pg/mL