Mouse IL-10 ELISA Set (without plates) (ab47599)
Overview
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Product name
Mouse IL-10 ELISA Set (without plates) -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
Range
31.25 pg/ml - 1000 pg/mlAssay duration
Multiple steps standard assaySpecies reactivity
Reacts with: MouseProduct overview
Abcam’s IL-10 Murine in vitro ELISA Set is designed for the quantitative measurement of Murine IL-10 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
The murine IL-10 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
This ELISA Set is intended to measure murine IL-10 in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
Recognizes both natural and recombinant Murine IL-10
Tested applications
Suitable for: Sandwich ELISAmore detailsPlatform
ReagentsProperties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 5 x 96 tests 10 x 96 tests 15 x 96 tests Detection biotinylated anti-murine IL-10 antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml Murine IL-10 Capture antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml Murine IL-10 standard 5 vials 10 vials 15 vials Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials Streptavidin-HRP 1 vial 2 vials 3 vials -
Research areas
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Function
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
Tissue specificity
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CSIF
- Cytokine synthesis inhibitory factor
- IL 10
see all -
Database links
- Entrez Gene: 16153 Mouse
- SwissProt: Q0VBJ1 Mouse
- SwissProt: P18893 Mouse
- Unigene: 874 Mouse
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 5 x 96 tests 10 x 96 tests 15 x 96 tests Detection biotinylated anti-murine IL-10 antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml Murine IL-10 Capture antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml Murine IL-10 standard 5 vials 10 vials 15 vials Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials Streptavidin-HRP 1 vial 2 vials 3 vials -
Research areas
-
Function
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
Tissue specificity
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- CSIF
- Cytokine synthesis inhibitory factor
- IL 10
see all -
Database links
- Entrez Gene: 16153 Mouse
- SwissProt: Q0VBJ1 Mouse
- SwissProt: P18893 Mouse
- Unigene: 874 Mouse
Images
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Murine IL-10 ELISA Set (ab47599)Murine IL-10 standard curve ranging from 31.25 to 1000 pg/mL