Rat IL-33 ELISA Kit (ab236714)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 6 pg/ml
- Range: 31.3 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat IL-33 ELISA Kit
See all IL-33 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 2.5% Inter-assay Sample n Mean SD CV% Serum 3 3% -
Sample type
Cell culture supernatant, Serum, Tissue Extracts, Hep Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
6 pg/ml -
Range
31.3 pg/ml - 3000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 117 106% - 123% Serum 99 98% - 101% Tissue Extracts 87 85% - 92% Cell culture media 117 110% - 121% Hep Plasma 82 81% - 83% Cit plasma 103 101% - 106% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Rat -
Product overview
Rat IL-33 ELISA Kit (ab236714) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-33 protein in cit plasma, hep plasma, serum, tissue extracts, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat IL-33 with 6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Rat IL-33 Capture Antibody 1 x 600µl 10X Rat IL-33 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BR 1 x 6ml Plate Seals 1 unit Rat IL-33 Lyophilized Recombinant Protein 2 vials Sample Diluent 50BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528).
In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets (PubMed:21734074). This form is rapidely lost upon angiogenic or proinflammatory activation (PubMed:18787100). -
Tissue specificity
Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta. -
Sequence similarities
Belongs to the IL-1 family. Highly divergent. -
Domain
The homeodomain-like HTH domain mediates nuclear localization and heterochromatin association. -
Post-translational
modificationsThe full length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein. May also be proteolytically processed by calpains (PubMed:19596270). Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation (PubMed:19559631). In vitro proteolytic cleavage by CASP1 was reported (PubMed:16286016) but could not be confirmed in vivo (PubMed:19465481) suggesting that IL33 is probably not a direct substrate for that caspase. -
Cellular localization
Nucleus. Chromosome. Cytoplasmic vesicle, secretory vesicle. Secreted. Associates with heterochromatin and mitotic chromosomes (PubMed:17185418). - Information by UniProt
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Alternative names
- C9orf26
- CHROMOSOME 9 OPEN READING FRAME 26
- DKFZp586H0523
see all -
Database links
- Entrez Gene: 361749 Rat
- SwissProt: Q66H70 Rat
Images
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Other - Rat IL-33 ELISA Kit (ab236714)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Rat Il-33 Standard Curve Comparison.Standard Curve comparison between Rat IL-33 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
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Example of rat IL-33 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of rat IL-33 standard curve in Sample Diluent 50BP.Background-subtracted data values (mean +/- SD) are graphed.
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Example of rat IL-33 standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native IL-33 in rat lung tissue culture supernatant samples and rat spleen tissue supernatant samples.Interpolated concentrations of native IL-33 in stimulated (LPS) and unstimulated (mock) 6 days rat lung tissue culture supernatant samples and stimulated and unstimulated 18 hours rat spleen tissue supernatant samples. The concentrations of IL-33 were measured in duplicates, interpolated from the IL-33 standard curves and corrected for sample dilution. Undiluted samples are as follows: lung (LPS) neat, lung (mock) neat, spleen (LPS) neat, and spleen (mock) neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-33 concentration was determined to be 931.1 pg/mL in LPS stimulated lung tissue supernatant, 940.4 pg/mL in unstimulated rat lung tissue supernatant, 1,503 pg/mL in LPS stimulated spleen tissue supernatant, and 1,185 pg/mL in unstimulated spleen tissue supernatant.
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Interpolated concentrations of native IL-33 in rat kidney tissue extract based on a 500 µg/mL extract load.The concentrations of IL-33 were measured in duplicate and interpolated from the IL-33 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-33 concentration was determined to be 464.4 pg/mL in kidney tissue extract.
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Comparison of IL-33 in unstimulated and LPS stimulated lung and spleen tissue supernatants.Lung tissue was cultured for 6 days in the presence or absence (mock) of 5 µg/mL LPS. Spleen tissue was cultured for 18 hours in the presence or absence (mock) of 1 µg/mL LPS. The concentrations of IL-33 were measured in three different dilutions of the supernatant samples in duplicates and interpolated from the IL-33 standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean IL-33 concentration was determined to be 965.8 pg/mL in LPS stimulated lung tissue culture supernatant, 940.4 in unstimulated lung tissue culture supernatant, 1,463 pg/mL in LPS stimulated spleen tissue culture supernatant, 1,165 pg/mL in unstimulated spleen tissue culture supernatant, and undetectable in RPMI1640 media containing 10% FBS (not shown).
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Sandwich ELISA - Rat IL-33 ELISA Kit (ab236714)To learn more about the advantages of recombinant antibodies see here.