Rat Haptoglobin ELISA Kit (ab108857)
Key features and details
- Sensitivity: 1.3 µg/ml
- Range: 1.875 µg/ml - 120 µg/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Rat
Overview
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Product name
Rat Haptoglobin ELISA Kit
See all Haptoglobin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.9% Inter-assay Sample n Mean SD CV% Overall 7.5% -
Sample type
Serum, Plasma -
Assay type
Competitive -
Sensitivity
= 1.3 µg/ml -
Range
1.875 µg/ml - 120 µg/ml -
Recovery
99 %
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Assay time
3h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat -
Product overview
Abcam’s Haptoglobin Rat in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Haptoglobin in plasma and serum.
A Haptoglobin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Haptoglobin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of haptoglobin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 1 x 30ml 4X Biotinylated Haptoglobulin (Lyophilized) 1 vial Chromogen Substrate 1 x 7ml Rat Haptoglobulin Microplate (12 x 8 well strips) 1 unit Rat Haptoglobulin Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
Research areas
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Function
As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Anhaptoglobinemia -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 2 Sushi (CCP/SCR) domains. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Binding peptide
- BP
- Haptoglobin alpha chain
see all -
Database links
- Entrez Gene: 24464 Rat
- SwissProt: P06866 Rat
- Unigene: 10950 Rat
Images
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Typical Standard Curve
Representative Standard Curve using ab108857.