Rat GM-CSF ELISA Kit (ab236709)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.1 pg/ml
- Range: 3.1 pg/ml - 250 pg/ml
- Sample type: Serum, Tissue Culture Media
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat GM-CSF ELISA Kit
See all GM-CSF kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 5 2.2% Inter-assay Sample n Mean SD CV% Supernatant 3 3.7% -
Sample type
Serum, Tissue Culture Media -
Assay type
Sandwich (quantitative) -
Sensitivity
1.1 pg/ml -
Range
3.1 pg/ml - 250 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 92 91% - 92% Tissue Culture Media 90 84% - 98% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Rat -
Product overview
Rat GM-CSF ELISA Kit (ab236709) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GM-CSF protein in serum and tissue culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat GM-CSF with 1.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Rat GM-CSF Capture Antibody 1 x 600µl 10X Rat GM-CSF Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPR 1 x 6ml Plate Seals 1 unit Rat GM-CSF Lyophilized Recombinant Protein 2 vials Sample Diluent 25BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. -
Sequence similarities
Belongs to the GM-CSF family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Colony stimulating factor
- Colony Stimulating Factor 2
- Colony stimulating factor 2 (granulocyte-macrophage)
see all -
Database links
- Entrez Gene: 116630 Rat
- SwissProt: P48750 Rat
- Unigene: 44285 Rat
Images
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Other - Rat GM-CSF ELISA Kit (ab236709)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Rat GM-CSF competitor Std Curve ComparisonStandard curve comparison between Rat GM-CSF SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of rat GM-CSF standard curve in Sample Diluent NS.The GM-CSF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of rat GM-CSF standard curve in Sample Diluent 10BS.The GM-CSF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native GM-CSF in rat lung supernatant samples.The concentrations of GM-CSF were measured in duplicates, interpolated from the GM-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows: rat lung supernatant 10% The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GM-CSF concentration was determined to be 1534 pg/mL in rat lung supernatant.
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Interpolated concentrations of native GM-CSF in rat spleen supernatant samples.Treated for three days with 50 ng/ml PMA and 500 ng/ml calcium ionomycin or untreated. The concentrations of GM-CSF were measured in duplicates, interpolated from the GM-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows: treated spleen supernatant 1% and untreated spleen supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GM-CSF concentration was determined to be 10.7 ng/mL in treated spleen supernatant and 150.6 pg/mL in untreated spleen supernatant samples.
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Sandwich ELISA - Rat GM-CSF ELISA Kit (ab236709)To learn more about the advantages of recombinant antibodies see here.
