Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human REC8 knockout A549 cell lysate (ab257633)

Overview

  • Product name

    Human REC8 knockout A549 cell lysate

  • Product overview


    Knockout cell lysate achieved by CRISPR/Cas9.

  • Parental Cell Line

    A549

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 95 bp deletion in exon6.

  • Passage number

  • Knockout validation

    Sanger Sequencing

  • Reconstitution notes

    To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

    *Usage of SDS sample buffer is not recommended with these lyophilized lysates.

  • Notes

    Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.

    User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

    Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
    See here for more information on knockout cell lysates.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Properties

Target

  • Function

    Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.

  • Tissue specificity

    Expressed in testis and thymus.

  • Sequence similarities

    Belongs to the rad21 family.

  • Post-translational
    modifications

    Phosphorylated.

  • Cellular localization

    Nucleus. Chromosome. In meiotic chromosomes, localized along axial elements in prophase from the leptotene to diplotene stages. At later prophase stages, diakinesis and metaphase I, localized along interstitial axes of chromosomes including both centromere and arm regions. No longer detected in arm regions in anaphase I but persists on centromere regions until metaphase II.
  • Information by UniProt

  • Alternative names

    • Cohesin Rec8p
    • Human homolog of rad21 S. pombe
    • Meiotic recombination and sister chromatid cohesion phosphoprotein of the rad21p family
    • Meiotic recombination protein REC8 homolog
    • Meiotic recombination protein REC8-like 1
    • rec8
    • REC8 homolog (yeast)
    • REC8 like 1
    • REC8_HUMAN
    • Rec8p
    • Recombination and sister chromatid cohesion protein homolog
    see all

Properties

Images

  • Sanger Sequencing - Human REC8 knockout A549 cell lysate (ab257633)
    Sanger Sequencing - Human REC8 knockout A549 cell lysate (ab257633)
    Homozygous: 95 bp deletion in exon6

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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