Rat CINC-1 ELISA Kit (CXCL1) (ab155462)
Key features and details
- Sensitivity: 0.05 ng/ml
- Range: 0.047 ng/ml - 15 ng/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat CINC-1 ELISA Kit (CXCL1) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Intra-assay Inter-assay Sample n Mean SD CV% Inter-assay -
Sample type
Cell culture supernatant, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.05 ng/ml -
Range
0.047 ng/ml - 15 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 112.5 99% - 122% Plasma 107.9 97% - 125% -
Assay time
5h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat -
Product overview
Abcam’s CINC-1 (CXCL-1) Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of rat CINC-1 in serum, plasma and cell culture supernatants. (Collect plasma using EDTA and heparin as an anticoagulant). Citrate is not recommended as anticoagulants for use in this assay. Rat CINC-1 concentration in normal plasma is very low and it may not be detected in this assay.
This assay employs an antibody specific for rat CINC-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and CINC-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat CINC-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CINC-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect the endogenous Rat CINC-1 in normal serum with ab155462, only in serum spiked with Rat CINC-1.
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Notes
Sensitivity of the assay is dependant on the Assay Diluent used. Please refer to Protocol Booklet for more details.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Biotinylated anti-rat CINC-1 2 vials CINC-1 Microplate (12 x 8 wells) 1 unit Recombinant Rat CINC-1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Database links
- Entrez Gene: 81503 Rat
- SwissProt: P14095 Rat
Images
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Typical Standard Curve
Representative standard curve using ab155462 - Assay Diluent D.
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Typical Standard CurveRepresentative standard curve using ab155462 - Assay Diluent B.