Antibodies, Proteins, Kits and Reagents for Life Science

Anti-BCA1 antibody [EPR19259-147] - BSA and Azide free (ab251265)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19259-147] to BCA1 - BSA and Azide free
Suitable for: IHC-P
Reacts with: Mouse

Product name

Anti-BCA1 antibody [EPR19259-147] - BSA and Azide free
See all BCA1 primary antibodies
Description

Rabbit monoclonal [EPR19259-147] to BCA1 - BSA and Azide free
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Mouse

See all applications and species data
Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
General notes
Ab251265 is the carrier-free version of ab199043. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251265 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Mouse

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Clonality

Monoclonal
Clone number

EPR19259-147
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCA1 antibody [EPR19259-147] - BSA and Azide free (ab251265)

This data was developed using ab199043, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling BCA1 with ab199043 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadic staining on mouse spleen (PMID: 17563386). Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCA1 antibody [EPR19259-147] - BSA and Azide free (ab251265)

This data was developed using ab199043, the same antibody clone in a different buffer formulation.Negative Control: Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling BCA1 with ab199043 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. No staining on mouse cerebral cortex (PMID: 17563386). Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-BCA1 antibody [EPR19259-147] - BSA and Azide free (ab251265)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com