Ral Activation Assay Kit (ab212012)
Key features and details
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
Ral Activation Assay Kit -
Sample type
Tissue, Adherent cells, Suspension cells -
Species reactivity
Reacts with: Mouse, Rat, Chicken, Dog, Human -
Product overview
Ral Activation Assay Kit (ab212012) utilizes RalBP1 PBD Agarose beads to selectively isolate and pull-down the active form of Ral (RalA and RalB) from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Ral is detected by western blot analysis using an anti-Ral A antibody, which detects the human, mouse, rat, chicken and dog protein.
Features: 1) non radioactive assay format; 2) fast results: 1 hour assay plus electrophoresis/blotting time; 3) includes Cdc42 positive control; 4) pink colored agarose beads for easy identification during washing and aspiration steps.
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Notes
Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways.
The Ral proteins (RalA and RalB) belong to the Ras superfamily. They share 85% identity with each other and 58% with the oncoproteins H-Ras, K-Ras and N-Ras. Both RalA and RalB are 24 kDa proteins that regulate a variety of biological response pathways including vesicle trafficking, oncogenesis, transcription regulation and cell morphology. Like other small GTPases, Ral regulates molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In their active (GTP-bound) state, Ral A and Ral B bind specifically to the protein-binding domain (PBD) of RalBP1 to control downstream signaling cascades.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 20 tests ab211181 - RalBP1 PBD Agarose Beads 1 x 200µl 100X GTPyS 1 x 20µl 100X GDP 1 x 20µl 5X Assay/Lysis Buffer 4 x 2ml Anti-Ral A Mouse monoclonal antibody 1 x 10µl GTPase immunoblot Positive Control 1 x 100µl -
Research areas
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Relevance
RALA, resorcylic acid lactone alpha, is a low molecular weight GTP binding protein belonging to the RAS family of onco proteins. RALA has a 50% amino acid homology with RAS and an 85% homology with RALB. GTP binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. -
Cellular localization
Cell Membrane -
Alternative names
- DRal
- Ral
- RalA protein
see all -
Database links
- Entrez Gene: 5898 Human
- Entrez Gene: 5899 Human
- Entrez Gene: 56044 Mouse
- Entrez Gene: 81757 Rat
- Omim: 179550 Human
- Omim: 179551 Human
- SwissProt: P11233 Human
- SwissProt: P11234 Human
see all
Images
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Ral Activation Assay Kit (ab212012)
RalA positive control (10 µL of 293 cell lysate at 0.5 mg/mL) detected by immunoblot.
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Ral Activation Assay Kit (ab212012)Ral activation assay. Lane 1: NIH 3T3 cell lysate loaded with GDP and incubated with RalBP1 PBD Agarose beads. Lane 2: NIH 3T3 cell lysate loaded with GTPγS and incubated with RalBP1 PBD Agarose beads.