Rac2 Activation Assay Kit (ab211162)
Key features and details
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
Rac2 Activation Assay Kit -
Sample type
Tissue, Adherent cells, Suspension cells -
Species reactivity
Reacts with: Mouse, Human -
Product overview
Rac2 Activation Assay Kit (ab211162) uses PAK1 PBD Agarose beads to selectively isolate and pull-down the active form of Rac from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Rac is detected by western blot analysis using an anti-Rac2 specific polyclonal antibody, which reacts with the human and mouse protein.
Features: 1) non radioactive assay format; 2) fast results: 1 hour assay plus electrophoresis/blotting time; 3) includes Cdc42 positive control; 4) pink colored agarose beads for easy identification during washing and aspiration steps.
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Notes
Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways.
Rac2 (Ras-Related C3 Botulinum Toxin Substrate 2), a 21 kDa protein, belongs to the family of Rho GTPases and regulates a variety of biological response pathways that include secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Like other small GTPases, Rac2 regulates molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In its active (GTP-bound) state, Rac2 binds specifically to the p21-binding domain (PBD) of p21-activated protein kinase (PAK) to control downstream signaling cascades.
Diseases associated with Rac2 include neutrophil immunodeficiency syndrome and chronic granulomatous disease.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 20 tests 5 tests ab211177 - PAK1 PBD Agarose Beads 1 x 800µl 1 x 200µl 100X GTPyS 1 x 50µl 1 x 20µl 100X GDP 1 x 50µl 1 x 20µl 5X Assay/Lysis Buffer 1 x 30ml 4 x 2ml Anti-rac2 Rabbit polyclonal antibody 1 x 40µl 1 x 10µl Rac2 immunoblot Positive Control 1 x 100µl 1 x 100µl -
Research areas
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Function
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase. -
Tissue specificity
Hematopoietic specific. -
Involvement in disease
Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome (NEUID) [MIM:608203]. -
Sequence similarities
Belongs to the small GTPase superfamily. Rho family. -
Cellular localization
Cytoplasm. Membrane-associated when activated. - Information by UniProt
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Alternative names
- EN 7
- EN-7
- EN7
see all -
Database links
- Entrez Gene: 5880 Human
- Entrez Gene: 19354 Mouse
- Omim: 602049 Human
- SwissProt: P15153 Human
- SwissProt: Q05144 Mouse
- Unigene: 517601 Human
- Unigene: 1972 Mouse
Images
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Rac2 Activation Assay Kit (ab211162)
Rac2 positive control (recombinant human Rac2 from E. coli, partially purified) detected by immunoblot.
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Rac2 Activation Assay Kit (ab211162)Dot blot that demonstrates specificity of anti-Rac2 rabbit antibody.