Rabbit specific HRP/DAB Detection IHC Detection Kit - Micro-polymer (ab236469)
Overview
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Product name
Rabbit specific HRP/DAB Detection IHC Detection Kit - Micro-polymer
See all HRP/DAB detection kit kits -
Detection method
Colorimetric -
Assay type
Micro-Polymer -
Product overview
This IHC kit is used to detect rabbit primary antibodies in immunohistochemistry (IHC). It uses an HRP micro-polymer secondary antibody for highly sensitive detection.
HRP micro-polymer methods are more sensitive than the traditional streptavidin-biotin method, and they do not require a blocking step for endogenous biotin.
This kit is an exact copy of EXPOSE Rabbit Specific HRP/DAB Detection IHC Kit ab80437, except that it uses the HRP micro-polymer antibody used during the validation of Abcam's antibodies in IHC. This HRP micro-polymer antibody was identified by our R&D team as giving the most sensitive and specific staining.
The kit includes ready-to-use hydrogen peroxide blocking reagent, protein blocking reagent, HRP micro-polymer conjugated goat anti-rabbit secondary antibody, and DAB chromogen/substrate.
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Notes
The previous version of this kit, the EXPOSE Rabbit Specific HRP/DAB Detection IHC Kit, was used by researchers to stain over 75,000 slides since 2012 (based on volumes of reagent sold).
Other IHC detection products
For alternative kits, see anti-Mouse / anti-Rabbit HRP-DAB Micro-polymer IHC Kit ab236466, and HRP-AEC micro-polymer IHC kits.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
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Tested applications
Suitable for: IHC-Pmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 15 ml 60 ml 50x DAB Chromogen 1 x 0.5ml 1 x 2ml DAB substrate 1 x 15ml 1 x 60ml Goat anti-rabbit HRP Conjugate 1 x 15ml 1 x 60ml Hydrogen Peroxide Block 1 x 15ml 1 x 60ml Protein block 1 x 15ml 1 x 60ml -
Research areas
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Relevance
HRP/DAB detection kit is a immunoenzymatic antigen detection system for immunohistochemistry techniques.
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific HRP/DAB Detection IHC Detection Kit - Micro-polymer (ab236469)ab236469 Rabbit Specific HRP-DAB IHC Detection Kit - Micro-Polymer on Human Tonsil staining CD3
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)ab209064 at 1:2000 staining TMEM119 antibody in mouse cerebrum tissue by immunohistochemistry (FFPE). Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling TMEM119 with ab209064 at 1/2000 dilution followed by the same HRP micro-polymer goat anti-rabbit secondary antibody used in this kit. Positive staining on glial cells in mouse cerebrum is observed. Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0) before commencing with IHC staining protocol. Counter stained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) Hall OJ et al. PLoS Pathog. 12:e1005840 (2016) Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunohistochemical analysis of mouse lung tissue labeling Ki67 detected with ab80437 (this kit is identical to ab80437, except that it uses the HRP micro-polymer secondary antibody used by Abcam's R&D team for antibody validation in IHC). Tissue sections were embedded in paraffin and were counterstained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) Selby MJ et al. PLoS One (2016)Immunohistochemical (frozen) analysis of MC38 tumor sections labeling CD3 with ab16669 at 1/400 dilution. Sections were fixed with acetone, treated with peroxidase block to quench endogenous peroxidase, and then further blocked with a 10% goat serum and 5% BSA solution. CD3 positive T cells were detected using ab80437 (this kit is identical to ab80437, except that it uses the HRP micro-polymer antibody used by Abcam's R&D team for antibody validation in IHC). Invasive margin (top) shows sections derived from the periphery of the tumor and interior (bottom) shows sections from within the tumor.
