Rabbit monoclonal [RM223] Anti-Mouse IgG2c Fc (Biotin) (ab238619)
Key features and details
- Rabbit monoclonal [RM223] Anti-Mouse IgG2c Fc (Biotin)
- Conjugation: Biotin
- Host species: Rabbit
- Isotype: IgG
- Suitable for: WB, ELISA, ICC
Overview
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Product name
Rabbit monoclonal [RM223] Anti-Mouse IgG2c Fc (Biotin)
See all IgG2c secondary antibodies -
Host species
Rabbit -
Target species
Mouse -
Specificity
ab238619 reacts to the Fc region of mouse IgG2c. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.
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Tested applications
Suitable for: WB, ELISA, ICCmore details -
Immunogen
Full length protein corresponding to Mouse IgG2c.
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Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM223 -
Isotype
IgG -
Research areas
Images
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All lanes : Rabbit monoclonal [RM223] Anti-Mouse IgG2c Fc (Biotin) (ab238619) at 0.5 µg/ml
Lane 1 : Non-reduced (-) mouse IgG2c
Lane 2 : Reduced (+) mouse IgG2cThis antibody reacts to non-reduced IgG2c.
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A titer ELISA of mouse IgG2c. The plate was coated with different amounts of mouse IgG2c. A serial dilution of ab238619 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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ELISA of IgG from BalB/C and C57BL6 shows ab238619 reacts to C57BL6 IgG containing IgG2c, and does not react to BalB/C IgG containing IgG2a. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab2308619 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.