Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CXCL11 antibody [EPR23814-16] - BSA and Azide free (ab279706)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23814-16] to CXCL11 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CXCL11 antibody [EPR23814-16] - BSA and Azide free
    See all CXCL11 primary antibodies

  • Description

    Rabbit monoclonal [EPR23814-16] to CXCL11 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt (Intra),ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: RAW 264.7 (treated with 200 ng/ml interferon-gamma for 4 h, then 300 ng/ml Brefeldin A (BFA) for 20 h) and RAW 264.7 (treated with 80 nM PMA for 24 h, then with 100 ng/ml LPS for 4 h, then 300 ng/ml Brefeldin A (BFA) for 3 h) whole cell lysates; Mouse pancreas tissue lysate.

  • General notes

    ab279706 is the carrier-free version of ab259863. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab279706 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23814-16

  • Isotype

    IgG

Images

  • Western blot - Anti-CXCL11 antibody [EPR23814-16] - BSA and Azide free (ab279706)
    Western blot - Anti-CXCL11 antibody [EPR23814-16] - BSA and Azide free (ab279706)
    All lanes : Anti-CXCL11 antibody [EPR23814-16] (ab259863) at 1/2000 dilution

    Lane 1 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 2 : RAW264.7 treated with 200 ng/ml interferon-gamma for 4 hours, then 300 ng/ml Brefeldin A (BFA) was added to the treated cells for 20 hours, whole cell lysate
    Lane 3 : RAW264.7 whole cell lysate
    Lane 4 : RAW264.7 treated with 80 nM PMA for 24 hours, then treated with 100 ng/ml LPS for 4 hours, then 300 ng/ml Brefeldin A (BFA) was added to the treated cells for 3 hours, whole cell lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 10 kDa
    Observed band size: 11 kDa why is the actual band size different from the predicted?



    This data was developed using 259863, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 26 seconds.

     

  • Western blot - Anti-CXCL11 antibody [EPR23814-16] - BSA and Azide free (ab279706)
    Western blot - Anti-CXCL11 antibody [EPR23814-16] - BSA and Azide free (ab279706)
    All lanes : Anti-CXCL11 antibody [EPR23814-16] (ab259863) at 1/2000 dilution

    Lane 1 : Mouse pancreas tissue lysate
    Lane 2 : Mouse skeletal muscle tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 10 kDa
    Observed band size: 11 kDa why is the actual band size different from the predicted?



    This data was developed using 259863, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: skeletal muscle.

    Exposure time: 26 seconds.

     

  • (ab279706)
    (ab279706)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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