Rabbit F(ab')2 Anti-Dog IgG H&L (HRP) (ab136759)
Key features and details
- Rabbit F(ab')2 polyclonal Secondary Antibody to Dog IgG - H&L (HRP)
- Conjugation: HRP
- Host species: Rabbit
- Isotype: IgG
- Suitable for: IHC-Fr, IHC-P, Flow Cyt, ICC, WB, ELISA
Overview
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Product name
Rabbit F(ab')2 Anti-Dog IgG H&L (HRP)
See all IgG secondary antibodies -
Description
Rabbit F(ab')2 polyclonal Secondary Antibody to Dog IgG - H&L (HRP) -
Host species
Rabbit -
Target species
Dog -
Tested applications
Suitable for: IHC-Fr, IHC-P, Flow Cyt, ICC, WB, ELISAmore details -
Immunogen
Highly purified whole immunoglobulin from dog
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituents: 50% Tris buffered saline, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab136759 is purified by affinity chromatography and is thus particularly specific. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Preparation of Substrate Solutions:
- ELISA: OPD (orthophenylene diamine): 0.4mg/ml in a 0.1M pH5 phosphate citrate buffer + 0.4µl/ml H2O2 (30% hydrogen peroxide solution). Incubation 10 minutes in darkness. Stop reaction with V/2 volume of 1M H2SO4. Reading at 492nm
- Immunocytochemistry: DAB (diamino-benzidine): 0.50mg/ml in a 0.1M pH7.4 TRIS/HCl buffer + 5µl/ml H2O2 (30% hydrogen peroxide solution). Incubation 10 minutes in darkness.
- Western Blot: 4CN (4-chloro-1naphtol): 25mg in 49.5ml of 0.1M pH 7.4 PBS + 5µl/ml 100% ethanol. Filter on filter paper. Add 25µl of 30% H2O2. Incubate 10 min at room temperature. -
Research areas