Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6735)
Key features and details
- Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Rabbit
- Suitable for: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Fr
Overview
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Product name
Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase)
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Rat -
Tested applications
Suitable for: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Frmore details -
Immunogen
Rat IgG whole molecule
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Conjugation
Alkaline Phosphatase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.87% Sodium chloride, 0.788% Tris HCl, 0.0095% Magnesium chloride, 0.001% Zinc chloride, 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads. -
Conjugation notes
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons) -
Clonality
Polyclonal -
General notes
Part of the AbExcel range. -
Research areas
Images
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Western blot - Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6735)All lanes : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/ml (Anti-Tubulin antibody [YL1/2] - Loading Control)
All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-2 : Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6735) at 1/2000 dilution (Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
Lanes 3-4 : Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6735) at 1/10000 dilution (Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
Lanes 5-6 : Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6735) at 1/20000 dilution (Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
Developed using the ECL technique.
Performed under reducing conditions.