Protein A Magnetic Beads (ab214286)
Key features and details
- Sample type: Cell Lysate, Serum
Overview
-
Product name
Protein A Magnetic Beads -
Sample type
Serum, Cell Lysate -
Species reactivity
Reacts with: Mouse, Rabbit, Horse, Guinea pig, Hamster, Cow, Human, Pig
Does not react with: Rat, Sheep, Goat, Chicken -
Product overview
Features:
Easy to use, high-binding capacity, non-adherent beads.
Support Characteristics: Paramagnetic, spherical, 6 % cross-linked agarose.
Ligand: Recombinant Protein A.
Particle Size: 75 – 150 µm.
Binding Capacity: Generally >25 mg human IgG/ml wet beads.
Working Temperature: Room temperature.
Storage Solution: PBS with 0.02% Sodium Azide.
Storage Temperature: 4 – 8 °C.
Applications:
Useful for immunoprecipitation and enrichment of IgG antibodies.
High affinity for Fc region of IgG antibodies from a variety of species.
Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3).
It also binds to total IgG from cow, guinea pig, hamster, horse, pig, and rabbit. Protein A has little affinity to chicken, goat, rat and sheep.
-
Notes
Description:
Protein A Magnetic Beads are prepared by covalently coupling Recombinant Protein A to 6% crosslinked magnetically beaded agarose. The coupling technique is optimized to give a high binding capacity for IgG. The capacity of IgG binding is generally greater than 25 mg of human IgG per ml of wet gel.
-
Tested applications
Suitable for: Purification, IPmore details
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 ml Protein A magnetic beads 1 x 1ml
