Proteasome 20S Activity Assay Kit (Fluorometric) (ab112154)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Proteasome 20S Activity Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Quantitative -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
ab112154 Proteasome 20S Activity Assay Kit is a homogeneous fluorescent assay that measures the chymotrypsin-like protease activity associated with the proteasome complex in cultured cells. ab112154 uses LLVY-R110 as a fluorogenic indicator for proteasome activities. Cleavage of LLVY-R110 by the proteasome generates strongly green fluorescent R110 that is monitored fluorometrically at 520-530 nm with excitation at 480-500 nm. The kit provides all the essential components with an optimized assay protocol. The assay is robust, and can be readily adapted for high-throughput assays to evaluate the proteasome activities or screen inhibitors in cultured cells or in solution. The assay can be performed in a convenient 96-well and 384-well fluorescence microtiter-plate format.
Visit our FAQs page for tips and troubleshooting. -
Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests Assay Buffer 1 x 10ml DMSO 1 x 100µl Proteasome LLVY-R110 Substrate Component A 1 vial -
Research areas
Images
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Functional Studies - Proteasome 20S Activity Assay Kit (Fluorometric) (ab112154)Detection of Proteasome Activity in Jurkat cells. Jurkat cells were seeded on the same day at 500,000 cells/90 µL/well in a 96-well black wall/clear bottom plate. The cells were treated with or without 50 mM H2O2 for 30 minutes. The proteasome assay loading solution (100 µL/well) was added and incubated in a 5% CO2, 37 °C incubator for 3 hours. The fluorescence intensity was measured at Ex/Em = 490/525.