Protease Inhibitor Cocktail EDTA Free (ab270055) protects protein extracts from aminopeptidases, metalloproteases, and serine, cysteine, and aspartic acid proteases. It is supplied as a 100X stock solution of protease inhibitors dissolved in DMSO. Ab270055 is a general-purpose reagent suitable for protease inhibition during protein extraction from mammalian tissues and cells, yeast and fungal cells, and bacteria.

ab270055 does not contain the chelating agent EDTA and therefore its effectiveness against metalloproteases is limited by comparison to protease preparations that do contain EDTA. It is compatible with techniques which require the absence of EDTA, such as isoelectric focusing and metal affinity column separations. For other applications, use ab271306, which includes EDTA. 

This product is manufactured by Expedeon, an Abcam company. It was previously called Proteoloc™ Protease Inhibitor Cocktail EDTA Free. Expedeon product code 42516 is the same as the 3 mL size of this product, 42517 is the same as the 30 mL size, and 44214 is the same as the 10 mL size.

Composition: 

AEBSF: Irreversible serine protease inhibitor. Inhibits chymotrypsin, kallikrein, plasmin, thrombin, and trypsin.

Aprotinin: Reversible serine protease inhibitor. Inhibits chymotrypsin, kallikrein, plasmin, trypsinogen, and urokinase.

Bestatin: Metalloprotease inhibitor selective for aminopeptidases. Inhibits activity of leucine aminopeptidase, aminopeptidase B, and triamino peptidase.

E-64: Irreversible inhibitor of cystine proteases. Inhibits calpain, papain, and cathespin B.

Leupeptin: Reversible inhibitor of serine and cystine proteases. Inhibits calpain, papain, trypsin, and cathespin B.

Pepstatin A: Reversible aspartic acid protease inhibitor. Inhibits pepsin, rennin, cathespin D, chymosin, and protease B.

Recommended procedure:

Allow product to thaw at room temperature. Agitate or vortex container to ensure uniform solution. ab270055 is provided as a 100X stock solution of protease inhibitors. Dilute product into desired lysis buffer to obtain a 1X concentration (5 mM). For example, dilute 100 μL ab270055 into 10 mL Protein Extraction Kit (ab270054). Proceed in accordance with extraction kit or lysis buffer protocol.