Protease Activity Assay Kit (ab111750)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture supernatant, Milk, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 500 pg/well
Overview
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Product name
Protease Activity Assay Kit
See all Protease kits -
Detection method
Fluorescent -
Sample type
Cell culture supernatant, Milk, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Enzyme activity -
Sensitivity
Assay time
1h 00mProduct overview
Abcam's Protease Activity Assay Kit is designed for the quantitative determination of proteases present in the protein sample. The assay uses fluorescein isothiocyanate (FITC)-labeled casein as a general protease substrate. The fluorescein label on the FITC-Casein is highly quenched. Upon digestion by proteases present in the sample the FITC-Casein substrate is cleaved into smaller peptides which abolishes the quenching of the fluorescence label. The fluorescence of the FITC-labeled peptide fragments is measured at Ex/Em = 485/530 nm. The kit is supplied with our Mass Spectrometry Grade (MSG), chemically stabilized Trypsin for use as a general protease control. However, other protease standard controls can also be used. This kit is easy to use and can detect Visit our FAQs page for tips and troubleshooting.
Notes
Proteases are naturally present in all organisms. These enzymes are involved in a multitude of physiological reactions from simple digestion of food proteins to highly regulated cascades. Proteases can either break specific peptide bonds (limited proteolysis), depending on the amino acid sequence of a protein, or break down a complete peptide to amino acids (unlimited proteolysis). The activity can be a destructive change (abolishing a protein's function), an activation of a function (preform to mature form) or it can be a signal in a signaling pathway.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.Platform
Microplate readerProperties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests FITC Standard (25 µM) Yellow cap 1 x 200µl Positive Control (lyophilized) Green cap 1 vial Protease Assay Buffer WM 1 x 25ml Protease Substrate (lyophilized) Red cap 1 vial -
Research areas
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests FITC Standard (25 µM) Yellow cap 1 x 200µl Positive Control (lyophilized) Green cap 1 vial Protease Assay Buffer WM 1 x 25ml Protease Substrate (lyophilized) Red cap 1 vial -
Research areas
Images
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Functional Studies - Protease Activity Assay Kit (ab111750)Positive control assayed using ab111750
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Functional Studies - Protease Activity Assay Kit (ab111750)Example Standard curve obtained using ab111750
