Rat IL-3 ELISA Kit
(ab277709)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 14.5 pg/ml
- Range: 34.4 pg/ml - 2200 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
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Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 8 7.3% Inter-assay Sample n Mean SD CV% Supernatant 4 10.3% -
Sample type
Cell culture supernatant, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
14.5 pg/ml -
Range
34.4 pg/ml - 2200 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 100 96% - 104% EDTA Plasma 85 81% - 89% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Rat -
Product overview
Rat IL-3 ELISA kit (ab277709) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Rat IL-3 protein in human plasma (EDTA) and tissue culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat IL-3 with 14.5 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Interleukin 3 (IL-3) is a secreted 166 amino acids long, glycosylated interleukin coded by the IL3 gene. Previous names reported for IL-3 are Hematopoietic growth factor, Mast cell growth factor, multi-potential colony-stimulating factor, and P-cell-stimulating factor. IL-3 has been found to stimulate a variety of cell activities such as; proliferation, differentiation, survival of pluripotent hematopoietic stem cells, and may be associated with neurologic disorders. IL-3 is expressed by activated T-cells, mast cells, and natural killer cells. IL-3 performs its biological action by binding to a transmembrane receptor complex consisting of IL-3RA, also known as CD123, and a signal inducing beta subunit, which also acts as a receptor for colony stimulating factor 2 and Interleukin 5.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Rat IL-3 Capture Antibody 1 x 600µl 10X Rat IL-3 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Plate Seals 1 unit Rat IL-3 Lyophilized Recombinant Protein 2 vials Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.
This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes. -
Tissue specificity
Activated T-cells, mast cells, natural killer cells. -
Sequence similarities
Belongs to the IL-3 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Colony stimulating factor multiple
- Hematopoietic growth factor
- IL 3
see all -
Database links
- Entrez Gene: 24495 Rat
- SwissProt: P04823 Rat
- Unigene: 10652 Rat
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of rat IL-3 standard curve in Sample Diluent NS. The IL-3 standard curve was prepared as described in Section 10.Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native IL-3 in rat splenocyte supernatant. Splenocytes were cultured in RPMI supplemented with 10% fetal calf serum, 2 mM L-glutamine, 100 U/mLCells were cultured for 4 days at 37°C in the presence of 10 ng/mL of Tissue plasminogen activator (TPA) and 10 ng/mL of IL-1а. The concentrations of IL-3 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: splenocyte supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 1,044 pg/mL in splenocyte supernatant.
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Recombinant Antibody BenefitsTo learn more about the advantages of recombinant antibodies see here.
