PPDK (Pyruvate, phosphate dikinase) Activity Assay Kit (Fluorometric/Colorimetric) (ab273318)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate
- Sample type: Cell culture extracts, Cell Lysate, Purified protein
Overview
-
Product name
PPDK (Pyruvate, phosphate dikinase) Activity Assay Kit (Fluorometric/Colorimetric) -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell culture extracts, Cell Lysate, Purified protein -
Assay type
Enzyme activity (quantitative) -
Assay duration
Multiple steps standard assay -
Product overview
Pyruvate, phosphate dikinase (PPDK) Activity Assay Kit (Fluorometric) (ab273318) provides a simple and rapid test for measuring specific activity of PPDK in bacterial, protozoan and plant lysates as well as protein preps. We utilize the ability of PPDK to interconvert the substrate into intermediate product detected by proprietary enzyme mix and probe. Generated stable product can be quantified by either colorimetric or fluorometric readout.
The assay is simple to perform, high-throughput adaptable and a fluorometric reaction can detect as low as of 0.006 U of PPDK activity in a single well. We provide sufficient reagents for 100 fluorometric or colorimetric assays.
-
Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests PPDK Assay Buffer 1 x 25ml PPDK Cofactor Mix 1 x 200µl PPDK Developer (Lyophilized) 1 vial PPDK Positive Control 1 x 50µl PPDK Probe 1 x 200µl PPDK Substrate Mix (Lyophilized) 1 vial Pyruvate Standard (100 mM) 1 x 100µl
Images
-
Example data
Typical standard curve: Colorimetric – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
-
Example dataQuantification of PPDK Activity in protein extracts; 40 µg of Leaf; 4.8 µg of E. coli expressing PPDK; 120 µg of Negative Control (untransformed E. coli strain). Activity was calculated according to the kit protocol using colorimetric method.
-
Example dataTypical standard curve: Fluorometric – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.