Porcine IL-12 ELISA Kit (ab273207)
Key features and details
- Sensitivity: 2.2 ng/ml
- Range: 2.458 ng/ml - 600 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Pig
Overview
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Product name
Porcine IL-12 ELISA Kit
See all IL-12 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.2 ng/ml -
Range
2.458 ng/ml - 600 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 78.43 68% - 81% Plasma 72.71 68% - 83% Cell culture media 98.1 68% - 130% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Pig -
Product overview
Porcine IL-12 ELISA Kit (ab273207) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Porcine IL-12 in serum (Porcine IL-12 concentration is low in normal serum/plasma and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Porcine IL-12 coated on a 96- well plate. Standards and samples are pipetted into the wells and IL-12 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti- Porcine IL-12 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-12 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 1800X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Anti-Porcine IL-12 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Porcine IL-12 Antibody 2 vials Porcine IL-12 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CLMF
- CLMF p35
- CLMF p40
see all
Images
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Porcine IL-12 ELISA Standard Curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Porcine IL-12 ELISA kit (ab273207) Standard curve.