Porcine CXCL9 ELISA Kit (ab273210)
Key features and details
- Sensitivity: 5 pg/ml
- Range: 5 pg/ml - 1400 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
Overview
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Product name
Porcine CXCL9 ELISA Kit
See all CXCL9 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
5 pg/ml -
Range
5 pg/ml - 1400 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 88.25 68% - 116% Plasma 105.4 78% - 139% Cell culture media 94.1 70% - 123% -
Assay duration
Multiple steps standard assay -
Product overview
Porcine CXCL9 ELISA Kit (ab273210) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of porcine CXCL9 in serum, plasma (collect plasma using EDTA or citrate as an anti-coagulant. Heparin is not recommended for use in this assay) and cell culture supernatants.
This assay employs an antibody specific for porcine CXCL9 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-porcine CXCL9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Porcine CXCL9 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Porcine CXCL9 Antibody 2 vials Porcine CXCL9 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-X-C motif chemokine 9
- chemokine (C-X-C motif) ligand 9
- CMK
see all
Images
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Example standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.
