Mouse CCL6 ELISA Kit
(ab277715)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.97 pg/ml
- Range: 6.25 pg/ml - 400 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
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Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.2% Inter-assay Sample n Mean SD CV% Serum 3 5.8% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.97 pg/ml -
Range
6.25 pg/ml - 400 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 110 104% - 116% Serum 115 112% - 116% Tissue Extracts 94 92% - 97% Hep Plasma 114 110% - 117% EDTA Plasma 112 106% - 118% Cit plasma 110 106% - 115% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse CCL6 ELISA kit (ab277715) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse CCL6 protein in mouse serum, plasma, cell culture supernatant, and cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CCL6 with .97 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
CCL6, identified only in rodents, is a small cytokine that belongs to the CC chemokine family in which plays important roles in the immune system such as wound healing, cellular differentiation, macrophage chemoattractant, angiogenesis, and inflammation. Studies have shown CCL6 to promote innate immunity through cell activation and recruitment as well potentially having a role in tumorigenic and metastatic behavior.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse CCL6 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Mouse CCL6 Capture Antibody (Lyophilized) 1 x 600µl Mouse CCL6 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Function
CCL6(22-95) and CCL6(23-95) are potent chemoattractants. -
Tissue specificity
Expressed in myelopoietic bone marrow cultures stimulated by GM-CSF. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-C motif chemokine 6
- c10
- CC chemokine C10
see all -
Database links
- Entrez Gene: 20305 Mouse
- SwissProt: P27784 Mouse
- Unigene: 137 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse CCL6 standard curve in Sample Diluent NS. The CCL6 standard curve was prepared as described in Section 10.Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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. Example of mouse CCL6 standard curve in 1X Cell Extraction Buffer PTR.The CCL6 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native or spike CCL6 in mouse serum, plasma and cell culture supernatant samples.The concentrations of CCL6 were measured in duplicates, interpolated from the CCL6 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:20, plasma (citrate) 1:80, plasma (EDTA) 1:80, plasma (heparin) 1:80, and spleen supernatant 1:40. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL6 concentration was determined to be 3,747 pg/mL in serum, 14,568 pg/mL in plasma (citrate), 20,533 pg/mL in plasma (EDTA), 18,616 pg/mL in plasma (heparin), and 9,852 pg/mL in spleen supernatant.
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Interpolated concentrations of native CCL6 in mouse spleen extract sample based on a 400 µg/mL extract load.The concentrations of CCL6 were measured in duplicate and interpolated from the CCL6 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL6 concentration was determined to be 125.6 pg/mL in spleen extract.
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Recombinant Antibody BenefitsTo learn more about the advantages of recombinant antibodies see here.
