Pig IL-17 ELISA Kit (ab193732)
Key features and details
- Sensitivity: 14 pg/ml
- Range: 14 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Horse, Cow, Pig
Overview
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Product name
Pig IL-17 ELISA Kit
See all IL-17A kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% overall Inter-assay Sample n Mean SD CV% overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
14 pg/ml -
Range
14 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum = 89.69 82% - 98% Plasma = 85.28 70% - 108% Cell culture media = 97.43 87% - 116% -
Assay duration
One step assay -
Species reactivity
Reacts with: Horse, Cow, Pig -
Product overview
Abcam’s IL-17 Pig ELISA Kit (ab193732) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of porcine IL-17 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for porcine IL-17 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures IL-17 present in the samples. The wells are washed and biotinylated anti-porcine IL-17 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of IL-17 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 1 x 15ml 900x HRP-Streptavidin Concentrate 1 x 200µl Biotinylated porcine IL-17A detection antibody (lyophilized) 2 vials porcine IL-17A standards (lyophilized) 2 vials Pre-coated porcine IL-17A Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts. -
Tissue specificity
Restricted to activated memory T-cells. -
Sequence similarities
Belongs to the IL-17 family. -
Post-translational
modificationsFound both in glycosylated and nonglycosylated forms. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CTLA 8
- CTLA-8
- CTLA8
see all -
Database links
- Entrez Gene: 282863 Cow
- Entrez Gene: 449530 Pig
- SwissProt: Q687Y7 Cow
- SwissProt: Q60I29 Pig
Images
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Example of typical Pig IL-17 standard curve
