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Cancer Tumor immunology Cytokines Interleukins

Anti-IL-28A antibody [6H9E6] (ab233754)

Anti-IL-28A antibody [6H9E6] (ab233754)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [6H9E6] to IL-28A
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-IL-28A antibody [6H9E6]
    See all IL-28A primary antibodies
  • Description

    Mouse monoclonal [6H9E6] to IL-28A
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human IL-28A aa 1 to the C-terminus. (Expressed in E.coli).
    Database link: Q8IZJ0

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Human IL28A (aa 1-200) recombinant protein; IL28A (aa 1-200)-hIgGFc transfected HEK-293 cell lysate. ICC/IF: HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    6H9E6
  • Isotype

    IgG1
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling

Images

  • Western blot - Anti-IL-28A antibody [6H9E6] (ab233754)
    Western blot - Anti-IL-28A antibody [6H9E6] (ab233754)
    Anti-IL-28A antibody [6H9E6] (ab233754) at 1/500 dilution + Human IL28A (aa 1-200) recombinant protein

    Predicted band size: 22 kDa



    Expected MW is 48.2 kDa.

  • Western blot - Anti-IL-28A antibody [6H9E6] (ab233754)
    Western blot - Anti-IL-28A antibody [6H9E6] (ab233754)
    All lanes : Anti-IL-28A antibody [6H9E6] (ab233754) at 1/500 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : IL28A (aa 1-200)-hIgGFc transfected HEK-293 cell lysate

    Predicted band size: 22 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-IL-28A antibody [6H9E6] (ab233754)
    Immunocytochemistry/ Immunofluorescence - Anti-IL-28A antibody [6H9E6] (ab233754)

    HeLa (human prostate adenocarcinoma cell line) cells stained for IL28A (green) using ab233754 at 1/200 dilution in ICC/IF.

    Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Applications: IHC-P, WB

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