Phospho-AMPK alpha 1 (S487) and Total AMPK alpha 1 ELISA Kit (ab279734) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated AMPK alpha 1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-AMPK alpha 1 and total AMPK alpha 1. An anti-pan AMPK alpha 1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and AMPK alpha 1 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho-AMPK alpha 1 (S487) antibody is added to detect phosphorylated AMPK alpha 1. In the remaining wells, biotinylated anti-pan-AMPK alpha 1 antibody is used to detect pan AMPK alpha 1. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of AMPK alpha 1 (S487) or pan AMPK alpha 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.