Phosphatidylinositol Assay Kit (Fluorometric) (ab252900)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Plasma, Serum, Tissue Homogenate
Overview
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Product name
Phosphatidylinositol Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Serum, Plasma, Tissue Homogenate -
Assay type
Quantitative -
Product overview
The Phosphatidylinositol Assay Kit (Fluorometric) (ab252900) utilizes specific enzymes to generate an intermediate that then reacts with a Probe, yielding a signal that can be quantified fluorometrically, and is proportional to the amount of Phosphatidylinositol (PI) present in the sample. The assay is capable of detecting as little as 15 pmols of phosphatidylinositol.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 100 tests PI Assay Buffer 1 x 25ml PI Developer 1 vial PI Enzyme Mix 1 vial PI Hydrolysis Mix 1 vial PI Standard 1 vial Probe (in DMSO) 1 x 300µl Sample Clean-Up Mix 1 vial
Images
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Phosphatidylinositol standard curve
Phosphatidylinositol standard curve.
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Assay specificity.Specificity of the assay. Various phospholipids were assayed according to protocol: Phosphatidic Acid (PA; 5 nmol), Phosphatidylcholine (PC; 2.5 nmol), Sphingomyelin (SM; 5 nmol), Phosphatidylserine (PS; 5 nmol), and Cardiolipin (CL, 1 nmol) and 500 pmol phosphatidylinositol (PI).
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PI concentration in various tissues.(a) PI concentration in lipid extract of differentiated adipocyte cell lysate. (b) PI concentration in lipid extracted brain tissue. (c) PI concentration in lipid extracted from pooled human serum.