Phosphatidylglycerol Assay Kit (Fluorometric) (ab273288)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Cell culture extracts, Tissue Extracts
Overview
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Product name
Phosphatidylglycerol Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Cell culture extracts, Tissue Extracts -
Assay type
Quantitative -
Assay duration
Multiple steps standard assay -
Product overview
Phosphatidylglycerol Assay Kit (Fluorometric) (ab273288) utilizes specific enzymes to generate an intermediate that reacts with a probe. This yields a signal that can be quantified fluorometrically and is proportional to the amount of Phosphatidylglycerol (PG) present in the sample, unaffected by the presence of phosphatidic acid, cardiolipin, or other lipids. When used as described, the assay is highly selective against other phospholipids and capable of detecting as little as 20 pmoles of phosphatidylglycerol.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests PG Assay Buffer 1 x 25ml PG Converter Mix (Lyophilized) 1 vial PG Enzyme Mix (Lyophilized) 1 vial PG Probe (in DMSO) 1 x 200µl PG Standard (0.5 µM) (Lyophilized) 1 vial
Images
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PG standard curve.
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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Determination of total PG concentration in lipid extracted from brain lysate. -
Determination of Phosphatidylglycerol in lipid extracted from lung tissue. -
Specificity of assay.Specificity of assay, when used as described, for Phosphatidylglycerol (PG) versus Phosphatidylethanolamine (PE), Phosphatidic Acid (PA), Phosphatidylinositol (PI), and cardiolipin (CL). The amount of phospholipid for each sample was 1 nmole.
