Phosphatidylethanolamine Assay Kit (ab241005)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate reader
Overview
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Product name
Phosphatidylethanolamine Assay Kit -
Detection method
Fluorescent -
Product overview
The Phosphatidylethanolamine assay kit (ab241005) is a microplate based enzymatic assay for the quantitation of PE in cells and tissues. PE Converter hydrolyses PE to an intermediate, which converts a colorless probe to a fluorescent product via enzymatic reaction (Ex/Em: 535/587).
The intermediate formed through PE converter hydrolysis is specific to phosphatidylethanolamine. Thus no other phospholipids (i.e. phosphatidylcholine, phosphatidylinositol or phosphatidic acid) will be detected, making the kit highly specific. This assay kit can detect as low as 0.2 nmol per well.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests PE Assay Buffer 1 x 25ml PE Converter Cap colour is white. 1 vial PE Developer 1 x 600µl PE Enzyme Mix 1 vial PE Probe 1 x 200µl PE Standard (1 mM) 1 x 100µl -
Relevance
Phosphatidylethanolamine (PE), also known as cephalin is the second most abundant phospholipid in animal and plant tissues, and is present on the cytoplasmic side of the plasma membrane. Phosphatidylethanolamine is a neutral phospholipid consisting of a phosphatidyl group ester linked to an ethanolamine molecule. Its functions include membrane fission/fusion, maintenance of membrane curvature and stabilization of membrane proteins, since it can form hydrogen bonds with proteins through an ionizable amine group. It acts as a chaperone during assembly of membrane proteins and aids in their translocation from the cytoplasm to the membrane. It is also involved in secretion of very low density lipoproteins in the liver.
Images
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Phosphatidylethanolamine standard curve
Phosphatidylethanolamine standard curve
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Phosphatidylethanolamine content in rat liver (100 µg wet tissue) and HEK 293 cells (25 µg protein).Phosphatidylethanolamine content in rat liver (100 µg wet tissue) and HEK 293 cells (25 µg protein). Sample preparation and assay was carried out according to kit protocol.