Phalloidin-iFluor 750 Reagent (ab176762)
Key features and details
- Assay type: Cell-based (qualitative)
- Platform: Fluorescence microscope
- Sample type: Adherent cells, Suspension cells
Overview
-
Product name
Phalloidin-iFluor 750 Reagent
See all F-actin kits -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (qualitative) -
Product overview
Phalloidin-iFluor 750 Reagent (ab176762) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. Phalloidin-iFluor 750 can be detected with a fluorescent microscope at Ex/Em = 752/778 nm (NIR range).
phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.
Phalloidin binds to actin filaments much more tightly than to actin monomers, leading to a decrease in the dissociation rate of actin subunits from filaments ends, essentially stabilizing actin filaments through the prevention of filament depolymerization.
-
Platform
Fluorescence microscope
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 300 tests Phalloidin-iFluor 750 Conjugate 1 x 300 tests -
Research areas
-
Alternative names
- actin filament
- f actin
- Filamentous actin
Images
-
CytoPainter Phalloidin-iFluor 750 Reagent (ab176762)
Excitation and emission spectra of phalloidin-iFluor 750 reagent.