Peroxynitrite Assay Kit (Fluorometric) (ab233469)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Fluorescent
- Platform: Microplate reader
Overview
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Product name
Peroxynitrite Assay Kit (Fluorometric)
See all Peroxynitrite kits -
Detection method
Fluorescent -
Assay type
Cell-based (quantitative) -
Product overview
Peroxynitrite Assay Kit (Fluorometric) (ab233469) provides a sensitive tool to measure peroxynitrite in solution.
Peroxynitrite Sensor Green reacts with ONOO¯ to generate a bright green fluorescent product. It specifically reacts with ONOO¯ with high selectivity over other reactive oxygen species (ROS) and reactive nitrogen species (RNS). This kit can be used with a fluorescence microplate reader and spectrometer.
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Notes
Peroxynitrite (ONOO¯) is a strong oxidizing species and a highly active nitrating agent. It is formed from the reaction between superoxide radicals and nitric oxide generated in cells. It can damage a wide array of biomolecules including proteins, enzymes, lipids and nucleic acids, eventually contributing to cell death. Due to its extremely short half-life and low steady-state concentration, it has been challenging to detect and quantify peroxynitrite in solution.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Assay Buffer 1 x 20ml DMSO 1 x 100µl Peroxynitrite Green reagent 1 vial
Images
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Fluorescence response of DAX-J2™ PON Green 99 to different reactive oxygen species.
The fluorescence intensities were measured with Ex/Em = 490/530 nm.
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Example standard curve of Peroxynitrite showing concentration against absorbance.Peroxynitrite was measured with the Peroxynitrite Quantification Kit (Fluorometric) (ab233469) on a solid black 96-well plate using a Gemini microplate reader. As low as 1.25 μM was detected. (Δ Note: The fluorescence background increases with time, thus it is important to subtract the fluorescence intensity value of the blank wells for each data point.)